Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis

ABNORMALLY SHORT LINK BETWEEN LYS A51 AND PLP A401

O-acetylserine sulfhydrylase B, O-acetylserine(Thiol)-lyase B, CSase B

RHMTRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIEQAEADGLLRPGATILEPT
SGNTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQIIFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANT
DSHYCGTGPELLADLPEITHFVAGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTA
RYSVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKYLSTGAYAGSLDDAETALE
GQLWA

MNVTVSIPTILRPHTGGQKSVSASGDTLGAVISDLEANYSGISERLMDPSSPGKLHRFVNIYVNDEDVRFSGGLATAIAD
GDSVTILPAVAGG

7-10% w/v PEG 4000, 0.1 M Sodium citrate pH 5.8, 0.2 M Ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.80

Crystal structure of Mycobacterium tuberculosis CysM, the cysteine synthase B

Crystal structure of Mycobacterium tuberculosis CysO, an antigen

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Cysteine synthase B (E.C.2.5.1.47), 9.5 kDa culture filtrate antigen cfp10A

The asymmetric unit contains the biological unit consisting of two CysM protomers and one CysO protomer.

Sulfur carrier protein complex, beta-grasp fold, Amino-acid biosynthesis, Cysteine biosynthesis, Pyridoxal phosphate, Transferase

MTRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIEQAEADGLLRPGATILEPTSG
NTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQIIFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANTDS
HYCGTGPELLADLPEITHFVAGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTARY
SVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKYLSTGAYAGSLDDAETALEGQ
LWA