Crystal structure of the Spinach Aquaporin SoPIP2;1 S274E mutant

Structural and functional analysis of SoPIP2;1 mutants adds insight into plant aquaporin gating.

MSHHHHHHSSGLVPRGSHMLEDLMSKEVSEEAQAHQHGKDYVDPPPAPFFDLGELKLWSFWRAAIAEFIATLLFLYITVA
TVIGHSKETVVCGSVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLLRALVYMIAQCLGAICGVGLV
KAFMKGPYNQFGGGANSVALGYNKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPILAPLPIGFAVFMVHLATIPIT
GTGINPARSFGAAVIFNSNKVWDDQWIFWVGPFIGAAVAAAYHQYVLRAAAIKALGEFRSNPTN

Reservoir: 0.1M Tris, 0.2M NaCl, 24% PEG400. Reservoir mixed 1:4 with 0.1M CdCl2, and then mixed 1:1 with protein, centrifuged briefly and supernatant used for crystallization, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 280K

Structure of of the SoPIP2;1 S115E mutant

Structure of of the SoPIP2;1 S274E:S115E mutant

membrane protein, aquaporin, Transmembrane, Transport, TRANSPORT PROTEIN

MSKEVSEEAQAHQHGKDYVDPPPAPFFDLGELKLWSFWRAAIAEFIATLLFLYITVATVIGHSKETVVCGSVGLLGIAWA
FGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLLRALVYMIAQCLGAICGVGLVKAFMKGPYNQFGGGANSVALGYN
KGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPILAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNSNKVWD
DQWIFWVGPFIGAAVAAAYHQYVLRAAAIKALGSFRSNPTN