Crystal structure of 5beta-reductase (AKR1D1) in complex with NADP+ and 4-androstenedione

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

Crystal Structures of Human Delta4-3-Ketosteroid 5beta-Reductase (AKR1D1) Reveal the Presence of an Alternative Binding Site Responsible for Substrate Inhibition (dagger) (,) (double dagger).

Delta(4)-3-ketosteroid 5-beta-reductase, Aldo-keto reductase family 1 member D1

MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIREKIAEGKVRRE
DIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKWLYHKSNLCATWEAMEACKDA
GLVKSLGVSNFNRRQLELILNKPGLKHKPVSNQVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLK
DALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEY
PFHDEY

PEG-4000 21%, 0.1M Tris, MPD 5% CaCl2 0.05M, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K

5b-reductase, 5beta-reductase, 5b-red, AKR1D1, AKR, aldo-keto reductase, NADP, androstenedione, NADPH, substrate inhibition, Bile acid catabolism, Cytoplasm, Disease mutation, Lipid metabolism, Oxidoreductase, Steroid metabolism