Crystal structure of putative acetyltransferase (YP_390128.1) from Desulfovibrio desulfuricans G20 at 2.28 A resolution

Joint Center for Structural Genomics (JCSG)

(MSE)GSDKIHHHHHHENLYFQG(MSE)TQLELLLERIIDRVNVNLRHQKFDVGDYVRRQTPHLHYSKFYAFYGLSADDP
VHFHFKNSSLAGSYLLGRVNVLHSALYKSDIRGDELKRKGQHFVCDGK(MSE)IPLHDDEVITIKDSFLNKTLVHSNSHD
PESPEEFTIRNTVA(MSE)PYANIHGSLTEGSFIGSFATVDLSTIHNSVVRYFSYVQTGELVGKCVEPGQIWIKSGDELE
FHYSFDKAILDKYISQEAGSCPTGVL(MSE)EFVEVRQEDFEEVFASGH(MSE)ASGAGSASGASVSGYAVIKGDTVIGE
NVLVSQRAYLDNAW(MSE)GKGSNAQENCYIINSRLERNCVTAHGGKIINAHLGD(MSE)IFTGFNSFLQGSESSPLKIG
DGCVV(MSE)PHTIIDLEEPLEIPAGHLVWGYIRNKADLAAHSISFEEFAKVDGEVT(MSE)GR(MSE)TFRGKGGPFLD
SFKKRIKRILSENGAFFDGSEGTGHAQKGKNFTFNIIQPYQDGDPRG(MSE)YPTILIQPNNGS

MGSDKIHHHHHHENLYFQGMTQLELLLERIIDRVNVNLRHQKFDVGDYVRRQTPHLHYSKFYAFYGLSADDPVHFHFKNS
SLAGSYLLGRVNVLHSALYKSDIRGDELKRKGQHFVCDGKMIPLHDDEVITIKDSFLNKTLVHSNSHDPESPEEFTIRNT
VAMPYANIHGSLTEGSFIGSFATVDLSTIHNSVVRYFSYVQTGELVGKCVEPGQIWIKSGDELEFHYSFDKAILDKYISQ
EAGSCPTGVLMEFVEVRQEDFEEVFASGHMASGAGSASGASVSGYAVIKGDTVIGENVLVSQRAYLDNAWMGKGSNAQEN
CYIINSRLERNCVTAHGGKIINAHLGDMIFTGFNSFLQGSESSPLKIGDGCVVMPHTIIDLEEPLEIPAGHLVWGYIRNK
ADLAAHSISFEEFAKVDGEVTMGRMTFRGKGGPFLDSFKKRIKRILSENGAFFDGSEGTGHAQKGKNFTFNIIQPYQDGD
PRGMYPTILIQPNNGS

Desulfovibrio desulfuricans subsp. desulfuricans str.

NANODROP, 0.2M K Formate, 20.0% PEG 3350, No Buffer pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K

THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG MGSDKIHHHHHHENLYFQG.

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
2. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE
INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY OF
THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75 FOR THE
REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION.
3. ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY.
4. EDO AND CL WERE MODELED BASED ON CRYSTALLIZATION CONDITIONS.
MG WAS TENTATIVELY MODELED BASED ON ENVIRONMENT.

YP_390128.1, Putative acetyltransferase, Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative, PSI-2, TRANSFERASE

MTQLELLLERIIDRVNVNLRHQKFDVGDYVRRQTPHLHYSKFYAFYGLSADDPVHFHFKNSSLAGSYLLGRVNVLHSALY
KSDIRGDELKRKGQHFVCDGKMIPLHDDEVITIKDSFLNKTLVHSNSHDPESPEEFTIRNTVAMPYANIHGSLTEGSFIG
SFATVDLSTIHNSVVRYFSYVQTGELVGKCVEPGQIWIKSGDELEFHYSFDKAILDKYISQEAGSCPTGVLMEFVEVRQE
DFEEVFASGHMASGAGSASGASVSGYAVIKGDTVIGENVLVSQRAYLDNAWMGKGSNAQENCYIINSRLERNCVTAHGGK
IINAHLGDMIFTGFNSFLQGSESSPLKIGDGCVVMPHTIIDLEEPLEIPAGHLVWGYIRNKADLAAHSISFEEFAKVDGE
VTMGRMTFRGKGGPFLDSFKKRIKRILSENGAFFDGSEGTGHAQKGKNFTFNIIQPYQDGDPRGMYPTILIQPNNGS