Crystal structure of the V. cholerae Histidine Kinase DctB Sensor Domain

Crystal Structures of C4-Dicarboxylate Ligand Complexes with Sensor Domains of Histidine Kinases DcuS and DctB.

(MSE)RFQYQALLNEHQSQLDRFSSHIVATLDKYAHIPHLISKDKELVDALLSAQNSAQIDITNRYLEQVNEVIQAADTY
LIDRFGNTIASSNWNLDRSFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAYPVIYAAEILGVIVVK(MSE)DL
SAIEQGWQNKSSYFVATDDHQVVF(MSE)SSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLGWQGDLQAEQSEWRK
PEKHWLQDDYIVSSRPLPELALTIRVLSPKIE

MRFQYQALLNEHQSQLDRFSSHIVATLDKYAHIPHLISKDKELVDALLSAQNSAQIDITNRYLEQVNEVIQAADTYLIDR
FGNTIASSNWNLDRSFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAYPVIYAAEILGVIVVKMDLSAIEQGWQ
NKSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLGWQGDLQAEQSEWRKPEKHWLQDDYIV
SSRPLPELALTIRVLSPKIE

isopropanol, calcium acetate, pH 7.5, vapor diffusion, temperature 277K

Crystal Structure of the E.coli DcuS Sensor Domain

Solvent flattening and Histogram matching

histidine kinase sensor domain, Phosphoprotein, Transferase, Two-component regulatory system

RFQYQALLNEHQSQLDRFSSHIVATLDKYAHIPHLISKDKELVDALLSAQNSAQIDITNRYLEQVNEVIQAADTYLIDRF
GNTIASSNWNLDRSFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAYPVIYAAEILGVIVVKMDLSAIEQGWQN
KSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLGWQGDLQAEQSEWRKPEKHWLQDDYIVS
SRPLPELALTIRVLSPKIE