The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase

The structure of a DnaB-family replicative helicase and its interactions with primase.

GSMNPEEKTVMLYNEYAEQAVLGSILTEPELIKECPLTPEHFSPGKHFNIYFTMQDLDRKGQSVDFTSIAARVGEKLPQL
GGFGYLTELAASVASTTTFKQHCQTVSEYFQKRKAISIAQQIIENVNESDDGPVKPIQEAVSELMEIEASGTDDDDGSID
EALVTVYEEIESADGNITGVPSGFTELDRMTYGYKRRNFVLIAARPSMGKTAFALKQAKNMSDNDDVVNLHSLEMGKKEN
IKRLIVTAGSINAQKIKAARRDFASEDWGKLSMAIGEISNSNINIFDKAGQSVNYIWSKTRQTKRKNPGKRVIVMIDYLQ
LLEPAKANDSRTNQISQISRDLKKMARELDVVVIALSQLSRQVEQRQDKRPMLSDLRESGQLEQDADIIEFLYRDDYYDK
ESESKNIVEVIIAKHRDGPVGTVSLAFIKEYGNFVNLERRFDDR

0.1 M Hepes (pH 7.5), 1 1.25 M MgAc, and 0.02 0.04% n-octylglucoside, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Hydrogens have been added in the riding positions.The bad geometry on ASN174 in chains A and F is due to low resolution. The structure is the best authors can do at this resolution with the currently available crystallography program.

MNPEEKTVMLYNEYAEQAVLGSILTEPELIKECPLTPEHFSPGKHFNIYFTMQDLDRKGQSVDFTSIAARVGEKLPQLGG
FGYLTELAASVASTTTFKQHCQTVSEYFQKRKAISIAQQIIENVNESDDGPVKPIQEAVSELMEIEASGTDDDDGSIDEA
LVTVYEEIESADGNITGVPSGFTELDRMTYGYKRRNFVLIAARPSMGKTAFALKQAKNMSDNDDVVNLHSLEMGKKENIK
RLIVTAGSINAQKIKAARRDFASEDWGKLSMAIGEISNSNINIFDKAGQSVNYIWSKTRQTKRKNPGKRVIVMIDYLQLL
EPAKANDSRTNQISQISRDLKKMARELDVVVIALSQLSRQVEQRQDKRPMLSDLRESGQLEQDADIIEFLYRDDYYDKES
ESKNIVEVIIAKHRDGPVGTVSLAFIKEYGNFVNLERRFDDR