Crystal structure of GDP-4-keto-6-deoxymannose-3-dehydratase (ColD) H188N mutant with bound GDP-perosamine

[(2R,3S,4R,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S,5S,6R)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-6-methyltetrahydro-2H-pyran-2-yl dihydrogen diphosphate

GDP-4-Keto-6-deoxy-D-mannose 3-Dehydratase, Accommodating a Sugar Substrate in the Active Site.

Pyridoxamine 5-phosphate-dependent dehydrase

GHMINYPLASSTWDDLEYKAIQSVLDSKMFTMGEYVKQYETQFAKTFGSKYAVMVSSGSTANLLMIAALFFTKKPRLKKG
DEIIVPAVSWSTTYYPLQQYGLRVKFVDIDINTLNIDIESLKEAVTDSTKAILTVNLLGNPNNFDEINKIIGGRDIILLE
DNCESMGATFNNKCAGTFGLMGTFSSFYSNHIATMEGGCIVTDDEEIYHILLCIRAHGWTRNLPKKNKVTGVKSDDQFEE
SFKFVLPGYNVRPLEMSGAIGIEQLKKLPRFISVRRKNAEYFLDKFKDHPYLDVQQETGESSWFGFSFIIKKDSGVIRKQ
LVENLNSAGIECRPIVTGNFLKNTDVLKYFDYTVHNNVDNAEYLDKNGLFVGNHQIELFDEIDYLREVLK

28% PEG 3400, 400 mM MgCl2, 100 mM MES, 2 mM 2-oxoglutarate, 10 mM GDP-perosamine, pH 6.0, batch, temperature 298K

Structure of GDP-4-keto-6-deoxymannose-3-dehydratase with a trapped PLP-glutamate geminal diamine

aspartate aminotransferase, colitose, perosamine, o-antigen, PLP, pyridoxal phosphate, TRANSFERASE

MINYPLASSTWDDLEYKAIQSVLDSKMFTMGEYVKQYETQFAKTFGSKYAVMVSSGSTANLLMIAALFFTKKPRLKKGDE
IIVPAVSWSTTYYPLQQYGLRVKFVDIDINTLNIDIESLKEAVTDSTKAILTVNLLGNPNNFDEINKIIGGRDIILLEDN
CESMGATFNNKCAGTFGLMGTFSSFYSHHIATMEGGCIVTDDEEIYHILLCIRAHGWTRNLPKKNKVTGVKSDDQFEESF
KFVLPGYNVRPLEMSGAIGIEQLKKLPRFISVRRKNAEYFLDKFKDHPYLDVQQETGESSWFGFSFIIKKDSGVIRKQLV
ENLNSAGIECRPIVTGNFLKNTDVLKYFDYTVHNNVDNAEYLDKNGLFVGNHQIELFDEIDYLREVLK