Crystal structure of pyrazolo pyrimidine derivative bound to the kinase domain of human LCK, (auto-phosphorylated on TYR394)

Ab initio fragment molecular orbital study of ligand binding to leukocyte-specific protein tyrosine (LCK) kinase

Proto-oncogene tyrosine-protein kinase LCK

LYMPHOCYTE KINASE, LCK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, LSK, T cell-specific protein-tyrosine kinase

QTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLY
AVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFG
LARLIEDNE(PTR)TAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMV
RPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP

QTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLY
AVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFG
LARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDN
CPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP

0.2M (NH4)2SO4, 0.1M SODIUM CACODYLATE, 30% PEG 8000, MPD 5.2%, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

STRUCTURAL BASIS FOR ACTIVATION OF HUMAN LYMPHOCYTE KINASE LCK UPON TYROSINE PHOSPHORYLATION NATURE VOL. 384, 484, 1996

TYROSINE-PROTEIN KINASE, ATP-BINDING, PHOSPHORYLATION, SIGNAL TRANSDUCTION, ALTERNATIVE SPLICING, KINASE, SH2 DOMAIN, SH3 DOMAIN, TRANSFERASE