Cefotaxime acyl-intermediate structure of class a beta-lacta Toho-1 E166A/R274N/R276N triple mutant

CEFOTAXIME, C3' cleaved, open, bound form

Structural Basis of Extend Spectrum Beta-Lactamase in Correlation of Enzymatic Kinetics and Thermal Stability of Acyl-Intermediates

ANSVQQQLEALEKSSGGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKQSESDKHLLNQRVEIKKSDLVNYN
PIAEKHVNGTMTLAELGAAALQYSDNTAMNKLIAHLGGPDKVTAFARSLGDETFRLDRTAPTLNTAIPGDPRDTTTPLAM
AQTLKNLTLGKALAETQRAQLVTWLKGNTTGSASIRAGLPKSWVVGDKTGSGDYGTTNDIAVIWPENHAPLVLVTYFTQP
EQKAENRNDILAAAAKIVTHGF

1.95-2.10M ammonium sulfate, 0.2M sodium citrate, PH5.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K

APO structure of Toho-1 E166A/R274N/R276N triple mutant

Apo structure of Toho-1 R274N/R276N double mutant

Cephalothin acyl-intermediate structure of Toho-1 E166A/R274N/R276N triple mutant

Aztreonam acyl-intermediate structure of Toho-1 E166A/R274N/R276N triple mutant

Ceftazidime acyl-intermediate structure of Toho-1 E166A/R274N/R276N triple mutant

EXTENDED-SPECTRUM, ESBL, BETA-LACTAMASE, ACYL-ENZYME, COMPLEX, CEFOTAXIME, TOHO-1, HYDROLASE, Antibiotic resistance

ANSVQQQLEALEKSSGGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKQSESDKHLLNQRVEIKKSDLVNYN
PIAEKHVNGTMTLAELGAAALQYSDNTAMNKLIAHLGGPDKVTAFARSLGDETFRLDRTEPTLNTAIPGDPRDTTTPLAM
AQTLKNLTLGKALAETQRAQLVTWLKGNTTGSASIRAGLPKSWVVGDKTGSGDYGTTNDIAVIWPENHAPLVLVTYFTQP
EQKAERRRDILAAAAKIVTHGF