Bovine pancreatic trypsin inhibitor (BPTI) containing only the [5,55] disulfide bond
Crystal structure of an extensively simplified variant of bovine pancreatic trypsin inhibitor in which over one-third of the residues are alanines
Basic protease inhibitor, BPTI, BPI, Aprotinin
Tris-HCl, pH8.7, Lithium sulfate, PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
NATIVELY FOLDED BPTI VARIANT ENGINEREED FROM THE WILD TYPE BPTI. THIS BPTI HAS A SINGLE DISSULFIDE BOND BETWEEN RESIDUE 5 AND 55, THE OTHER CYSTEINES BEING MUTATED TO A, G OR V. ADDITIONALLY, M52 WAS MUTATED TO L FOR PURIFICATION PURPOSE. THIS BPTI SEQUENCE CONTAINS A TOTAL OF 5 MUTATIONS (C14G, C30A, C38A, M52L AND C51A) WITH RESPECT TO THE WILD-TYPE SEQUENCE.
ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF)
sequence simplification, HYDROLASE INHIBITOR, Disulfide bond, Pharmaceutical, Protease inhibitor, Secreted, Serine protease inhibitor