Crystal structure of bleomycin-binding protein from Streptoalloteichus hindustanus complexed with bleomycin derivative

[(2S,3R,4R,5S,6S)-2-[(3R,4R,5R,6R)-2-[(1R,2S)-2-[[6-amino-2-[(1S)-3-amino-1-[(2,3-diamino-3-oxopropyl)amino]-3-oxopropyl]-5-methylpyrimidine-4-carbonyl]amino]-3-[[(2S,3R,4R)-5-[[(2R,3S)-1-[2-[4-[4-[3-[4-(3-aminopropylamino)butylamino]propylcarbamoyl]-1,3-thiazol-2-yl]-1,3-thiazol-2-yl]ethylamino]-3-hydroxy-1-oxobutan-2-yl]amino]-3-hydroxy-4-methyl-5-oxopentan-2-yl]amino]-1-(3H-imidazol-4-yl)-3-oxopropoxy]-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl] carbamate

Structure-Affinity Relationship Study of Bleomycins and Shble protein Using a Chemical Array

bleomycin-binding protein, BRP, Phleomycin resistance protein

MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSE
VVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD

PEG550mme, zinc sulfate, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K

thermostable mutant of shble complexed with bleomycin A2

ANTIBIOTIC RESISTANCE, bleomycin, Ligand binding protein, ANTIBIOTIC INHIBITOR