Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR

A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria.

Anti-Sigma factor ChrR, transcriptional activator ChrR

GPH(MSE)TDKSDRTDWVAL(MSE)RAIRDHRDEAAFAELFQHFAPKVKGFL(MSE)KSGSVASQAEECAQDV(MSE)AT
VWQKAHLFDPSRASVATWIFTIARNRRIDGLRKDRQPEPEDLFWGPDSEPDQADVYE(MSE)QQENARLGRAIARLPEAQ
RALIERAFFGDLTHRELAAETGLPLGTIKSRIRLALDRLRQH(MSE)S

GPHMTDKSDRTDWVALMRAIRDHRDEAAFAELFQHFAPKVKGFLMKSGSVASQAEECAQDVMATVWQKAHLFDPSRASVA
TWIFTIARNRRIDGLRKDRQPEPEDLFWGPDSEPDQADVYEMQQENARLGRAIARLPEAQRALIERAFFGDLTHRELAAE
TGLPLGTIKSRIRLALDRLRQHMS

(MSE)TIRHHVSDALLTAYAAGTLSEAFSLVVATHLSLCDECRARAGALDAVGGSL(MSE)EETAPVALSEGSLASV
(MSE)AQLDRQIQRPAPARRADPRAPAPLADYVGRRLEDVRWRTLGGGVRQAILPTGGEAIARLLWIPGGQAVPDHGHRG
LELTLVLQGAFRDETDRFGAGDIEIADQELEHTPVAERGLDCICLAATDAPLRFNSFL

MTIRHHVSDALLTAYAAGTLSEAFSLVVATHLSLCDECRARAGALDAVGGSLMEETAPVALSEGSLASVMAQLDRQIQRP
APARRADPRAPAPLADYVGRRLEDVRWRTLGGGVRQAILPTGGEAIARLLWIPGGQAVPDHGHRGLELTLVLQGAFRDET
DRFGAGDIEIADQELEHTPVAERGLDCICLAATDAPLRFNSFL

0.1 M MES, 0.2 M ammonium sulfate, 14-18% polyethylene glycol monomethylether 5000 (PEG5KMME), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K

Same complex but a different space group and slightly longer construct of ChrR

Sigma24, RpoE, Transcriptional activator chrR

ECF sigma factor, anti-sigma factor, cupin fold, DNA-binding, Transcription, Transcription regulation, Activator, Metal-binding, Zinc binding transcription factor

MTDKSDRTDWVALMRAIRDHRDEAAFAELFQHFAPKVKGFLMKSGSVASQAEECAQDVMATVWQKAHLFDPSRASVATWI
FTIARNRRIDGLRKDRQPEPEDLFWGPDSEPDQADVYEMQQENARLGRAIARLPEAQRALIERAFFGDLTHRELAAETGL
PLGTIKSRIRLALDRLRQHMS