Crystal structure of selenophosphate synthetase from Aquifex aeolicus complexed with AMPCPP

RIKEN Structural Genomics/Proteomics Initiative (RSGI)

DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER

Structure of selenophosphate synthetase essential for selenium incorporation into proteins and RNAs

Selenophosphate synthetase, Selenium donor protein

GSGGIEGRHMVELLKLVRSSGCAAKVGPGDLQEILKGFNIYTDESTLVSIGDDAGVYEHNGIIWVYTVDIITPVVNDPYL
WGAISTANALSDVYAMGGIPVNALAISCFNNCELDIEIFREVIRGALDKLREAKTVLLGGHTIDDKEPKFGLSVAGICPE
GKYITQSGAQVGQLLILTKPIGTGILIKGLKEGILKEEDINEAIENMLALNDKARNLMLSLDATACTDVTGFGLLGHAWN
ICKNSNIGARIFFEKVPYYQLSENLVKKKIYPKGAIENLNFVKNYLKSNLDNWKLILLSDPVTSGGLLFTINKEKLEKID
ETAKELEVNYWIIGETIAENVLEVL

17% PEG8000, 50mM KH2PO4, 0.1M MES, 1mM AMPCPP, 5mM CoCl2, pH 6.4, VAPOR DIFFUSION, temperature 293K

RIKEN Structural Genomics/Proteomics Initiative
NPPSFA, National Project on Protein Structural and Functional Analyses

An N-terminally truncated fragment, corresponding to residues 26-336 of the full-length protein.

An N-terminally truncated fragment, corresponding to residues 26-336 of the full-length SPS

We replaced selenocysteine 13 by Cys to facilitate the protein expression

Full-length selenophosphate synthetase complex with ATP-analog, Structural Genomics, NPPSFA, National Project on Protein Structural and Functional Analyses, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Transferase

MVELLKLVRSSGCAAKVGPGDLQEILKGFNIYTDESTLVSIGDDAGVYEHNGIIWVYTVDIITPVVNDPYLWGAISTANA
LSDVYAMGGIPVNALAISCFNNCELDIEIFREVIRGALDKLREAKTVLLGGHTIDDKEPKFGLSVAGICPEGKYITQSGA
QVGQLLILTKPIGTGILIKGLKEGILKEEDINEAIENMLALNDKARNLMLSLDATACTDVTGFGLLGHAWNICKNSNIGA
RIFFEKVPYYQLSENLVKKKIYPKGAIENLNFVKNYLKSNLDNWKLILLSDPVTSGGLLFTINKEKLEKIDETAKELEVN
YWIIGETIAENVLEVL