Crystal structure of Humanized HYHEL-10 FV mutant(HQ39KW47Y)-HEN lysozyme complex

Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10

1,4-beta-N-acetylmuramidase C, Allergen Gal d 4, Gal d IV

EIVMTQSPATLSVSPGERATLSCRASQSIGNNLHWYQQKPGQAPRLLIYYASQSISGIPARFSGSGSGTEFTLTISSLQS
EDFAVYYCQQSNSWPYTFGGGTKVEIK

QVQLQESGPGLMKPSETLSLTCSVSGDSIRSDYWSWIRKPPGKGLEYIGYVSYSGSTYYNPSLKSRVTISVDTSKNRFSL
KLNSVTAADTAVYYCARWDGDYWGQGILVTVSS

KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPC
SALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL

28-31% PEG MME 550, 0.01M zinc sulfate, 0.1M MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

humanized HYHEL-10 fv mutant(HW47Y)-hen lysozyme complex

humanized HyHEL-10 FV-HEN lysozyme complex

THERE IS NO UNP REFERENCE SEQUENCE DATABASE FOR ANTI- LYSOZYME ANTIBODY AT THE TIME OF PROCESSING. FOR VH FRAGMENT, 39TH RESIDUE, GLN AND 47TH RESIDUE, TRP WAS ENGINEERED TO LYS AND TYR, RESPECTIVELY.

IMMUNE SYSTEM, HYDROLASE, IMMUNE SYSTEM-HYDROLASE COMPLEX