Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA)

Potential Regulatory Interactions of Escherichia Coli Rraa Protein with Dead-Box Helicases.

MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFEDNGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELAR
LAVQNEWEGLVIYGAVRQVDDLEELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
E

RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNCYLEGEMVQGKRNEAIKRLTEGR
VNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLHRIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDE
LRPKTRAPSE

CRYSTALS WERE PREPARED USING THE HANGING DROP METHOD AT 16 DEG. C BY MIXING 1:1 PROTEIN SAMPLE WITH MOTHER LIQUOR: 100 MM MAGNESIUM ACETATE, 100 MM MOPS PH 7.2 AND 12% W/V PEG 8000.THE CRYSTALS WERE TRANSFERRED BRIEFLY TO RESERVOIR SOLUTION SUPPLEMENTED WITH 25% (V/V) GLYCEROL AND FLASH FROZEN IN LIQUID NITROGEN.

CRYSTAL STRUCTURE OF E. COLI REGULATOR OF RIBONUCLEASE ACTIVITY A (RRAA) BOUND TO FRAGMENT OF DEAD-BOX PROTEIN RHLB

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

HYDROLASE INHIBITOR-HYDROLASE COMPLEX, DEAD BOX RNA HELICASES