Solution structure of the closed conformation of human U2AF65 tandem RRM1 and RRM2 domains
Multi-Domain Conformational Selection Underlies Pre-Mrna Splicing Regulation by U2Af
TANDEM RRM1 AND RRM2 DOMAINS JOINED BY NATIVE LINKER, RESIDUES 148-342
U2 AUXILIARY FACTOR 65 KDA SUBUNIT, HUMAN U2AF65, U2 SNRNP AUXILIARY FACTOR LARGE SUBUNIT, HU2AF(65)
GAMARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSL KIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAF CEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNA
SOLUTION STRUCTURE OF THE SECOND RIBONUCLEIC ACID- BINDING DOMAIN OF HU2AF65
SOLUTION STRUCTURE OF THE FIRST RIBONUCLEIC ACID-BINDING DOMAIN OF HU2AF65
SOLUTION STRUCTURE OF THE THIRD RNA RECOGNITION MOTIF ( RRM)OF U2AF65 IN COMPLEX WITH AN N-TERMINAL SF1 PEPTIDE
STRUCTURE OF U2AF65 VARIANT WITH POLYURIDINE TRACT
X-RAY STRUCTURE OF A CORE U2AF65/U2AF35 HETERODIMER
SOLUTION STRUCTURE OF THE OPEN CONFORMATION OF HUMAN U2AF65 TANDEM RRM1 AND RRM2 DOMAINS
MODEL OF HUMAN U2AF65 TANDEM RRM1 AND RRM2 DOMAINS WITH EIGHT-SITE URIDINE BINDING
FINAL WATER REFINEMENT. THE STRUCTURE WAS DETERMINED USING A MULTI-STEP PROTOCOL. FIRST WITH REFINEMENT OF INDIVIDUAL STRUCTURED DOMAINS FOR RRM1 AND RRM2 OF U2AF65. THE INITIAL COORDINATES OF THE ISOLATED DOMAINS WERE BASED ON PDB ID 2G4B BY SICKMIER ET AL. 2006. MOL. CELL 23, 49- 59. SECOND STEP IS ADDITION AND RANDOMIZATION OF FLEXIBLE AND LINKER RESIDUES. THE THIRD STEP IS MODEL CALCULATION USING RDC ORIENTATION, PRE-BASED DISTANCE, TALOS DIHEDRAL AND HYDROGEN BOND RESTRAINTS WITH THE RRM1 AND RRM2 DOMAINS RESTRAINED TO THEIR INITIAL STARTING STRUCTURES. STRUCTURE CALCULATION INCLUDED DISTANCE RESTRAINTS BASED ON PARAMAGNETIC RELAXATION ENHANCEMENT DATA FROM SEVEN INDEPENDENT CYSTEINE MUTANTS (N155C, A164C, A171C, T209C, D273C, A287C AND A318C) COVALENTLY MODIFIED BY 3-(2- IODOACETAMIDO)-2,2,5,5, TETRAMETHYL-1- PYRROLIDINYLOXY RADICAL (IODOACETAMIDO-PROXYL).
BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ, RICE,SIMONSON,WARREN
PRE-MRNA SPLICING, TRANSCRIPTION, RNA BINDING PROTEIN, MRNA PROCESSING