Molecular Recognition of Chymotrypsin by the Serine Protease Inhibitor Ecotin from Yersinia pestis

CGVPAIQPVLSGLSRIVNGEEAVPGSWPWQVSLQDKTGFHFCGGSLINENWVVTAAHCGVTTSDVVVAGEFDQGSSSEKI
QKLKIAKVFKNSKYNSLTINNDITLLKLSTAASFSQTVSAVCLPSASDDFAAGTTCVTTGWGLTRYTNANTPDRLQQASL
PLLSNTNCKKYWGTKIKDAMICAGASGVSSCMGDSGGPLVCKKNGAWTLVGIVSWGSSTCSTSTPGVYARVTALVNWVQQ
TLAAN

DTPTPLNQQQPLEKIAPYPQAEKGMSRQVIFLEPQKDESRFKVELLIGKTLNVDCNRHMLGGNLETRTLSGWGFDYLVMD
KISQPASTMMACPEDSKPQVKFVTANLGDAAMQRYNSRLPIVVYVPQGVEVKYRIWEAGEDIRSAQVK

ACTIVATED BOVINE CHYMOTRYPSIN WAS PURCHASED FROM SIGMA-ALDRICH

RESERVOIR SOLUTION - 100 MM BIS TRIS PH 5.5, 200 MM AMMONIUM SULPHATE, 17.5% PEG3350. PROTEIN SOLUTION - 3 MG/ML ECOTIN, 6 MG/ML CHYMOTRYPSIN IN 5MM TRIS PH 7.5. DROP - 1:1 VOLUME RATIO RESERVOIR:PROTEIN.

GAMMA-CHYMOTRYPSIN D-NAPHTHYL-1-ACETAMIDO BORONIC ACID INHIBITOR COMPLEX

GAMMA CHYMOTRYPSIN COMPLEXED WITH DIETHYL PHOSPHORYL (PH 5.6, TEMPERATURE 90K)

BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION

GAMMA CHYMOTRYPSIN COMPLEXED WITH DIISOPROPYLPHOSPHOROFLUORIDATE

GAMMA CHYMOTRYPSIN COMPLEX WITH GLY-ALA-TRP

CRYSTAL STRUCTURE OF THE P1 THR BPTI MUTANT- BOVINECHYMOTRYPSIN COMPLEX

CRYSTAL STUCTURE OF GAMMA CHYMOTRYPSIN WITH N-ACETYL-PHENYLALANINE TRIFLUOROMETHYL KETONE BOUND AT THE ACTIVESITE

CRYSTAL STRUCTURE OF THE P1 TRP BPTI MUTANT- BOVINECHYMOTRYPSIN COMPLEX

STRUCTURAL CONSEQUENCES OF ACCOMMODATION OF FOUR NON-COGNATE AMINO-ACID RESIDUES IN THE S1 POCKET OF BOVINETRYPSIN AND CHYMOTRYPSIN

CRYSTAL STRUCTURE OF BOVINE GAMMA-CHYMOTRYPSIN

STRUCTURE OF THE COMPLEX BETWEEN BOVINE ALPHA-CHYMOTRYPSIN AND PMP-C, AN INHIBITOR FROM THE INSECT LOCUSTA MIGRATORIA

GAMMA-CHYMOTRYPSIN D-PARA-CHLORO-1-ACETAMIDO BORONIC ACID INHIBITOR COMPLEX

ALPHA-CHYMOTRYPSINOGEN COMPLEX WITH HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR VARIANT 4

ALPHA-CHYMOTRYPSIN COMPLEX WITH TURKEY OVOMUCOID THIRD DOMAIN (OMTKY3)

GAMMA CHYMOTRYPSIN ALKYLATED WITH N-ACETYL-L -ALANYL-L-PHENYLALANYL-ALPHA-CHLOROETHYLKETONE

GAMMA CHYMOTRYPSIN COMPLEXED WITH N-ACETYL D -TRYPTOPHAN

GAMMA CHYMOTRYPSIN WITH N-ACETYL-L- PHENYLALANYL TRIFLUOROMETHYL KETONE BOUND AT THE ACTIVE SITE

CRYSTAL STRUCTURE OF GAMMA-CHYMOTRYPSIN IN COMPLEX WITH 7-HYDROXYCOUMARIN

GAMMA CHYMOTRYPSIN IN 4% AQUEOUS SOLUTION OF ISOPROPANOL

GAMMA-CHYMOTRYPSIN L-NAPHTHYL-1-ACETAMIDO BORONIC ACID ACID INHIBITOR COMPLEX

GAMMA CHYMOTRYPSIN WITH N-ACETYL-L-LEUCYL- L-PHENYLALANYL TRIFLUOROMETHYL KETONE BOUND AT THE ACTIVE SITE

GAMMA CHYMOTRYPSIN COMPLEX WITH TRANS-O- HYDROXY-ALPHA-METHYL CINNAMATE

ALPHA-CHYMOTRYPSINOGEN COMPLEX WITH HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR VARIANT 3

GAMMA CHYMOTRYPSIN COMPLEX WITH P-DIETHYLAMINO -O-HYDROXY-ALPHA-METHYL CINNAMATE

CRYSTAL STRUCTURE OF BOVINE GAMMA-CHYMOTRYPSIN COMPLEXED WITH A SYNTHETIC INHIBITOR

CRYSTAL STRUCTURE OF A COMPLEX FORMED BETWEEN ORGANICSOLVENT TREATED BOVINE ALPHA- CHYMOTRYPSIN AND ITSAUTOCATALYTICALLY PRODUCED HIGHLY POTENT 14-RESIDUEPEPTIDE AT 2.2 RESOLUTION

STRUCTURE OF THE COMPLEX BETWEEN BOVINE ALPHA-CHYMOTRYPSIN AND PMP-D2V, AN INHIBITOR FROM THE INSECT LOCUSTA MIGRATORIA

CRYSTAL STUCTURE OF GAMMA CHYMOTRYPSIN WITH N-ACETYL-LEUCIL-PHENYLALANINE ALDEHYDE BOUND AT THE ACTIVE SITE

CRYSTAL STRUCTURE OF THE P1 GLU BPTI MUTANT- BOVINECHYMOTRYPSIN COMPLEX

HIGH RESOLUTION STRUCTURE OF BOVINE ALPHA- CHYMOTRYPSIN

CRYSTAL STRUCTURE ANALYSIS OF DELTA- CHYMOTRYPSIN BOUND TO A PEPTIDYL CHLOROMETHYL KETONE INHIBITOR

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN BOVINE CHYMOTRYPSINAND ECOTIN

CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINECHYMOTRYPSIN COMPLEX

PHOTOLYSIS PRODUCT OF P-DIETHYLAMINO-O- HYDROXY-ALPHA-METHYL CINNAMATE INHIBITED GAMMA CHYMOTRYPSIN

CRYSTAL STRUCTURE OF BOVINE CHYMOTRYPSINOGEN A (TETRAGONAL)

GAMMA-CHYMOTRYPSIN L-PARA-CHLORO-1-ACETAMIDO BORONIC ACID INHIBITOR COMPLEX

CRYSTAL STRUCTURE OF THE P1 MET BPTI MUTANT- BOVINECHYMOTRYPSIN COMPLEX

LYS 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH ALPHA-CHYMOTRYPSIN

ALPHA CHYMOTRYPSIN A COMPLEX WITH PHENYLETHANE BORONIC ACID (PEBA)

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AG" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY
A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.
THE SHEETS PRESENTED AS "BB" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY
A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.
THE SHEETS PRESENTED AS "CB" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY
A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.
THE SHEETS PRESENTED AS "DF" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY
A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.