X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and Man5(D1)

Structural Basis of Flocculin-Mediated Social Behavior in Yeast

LECTIN-LIKE FLO5A-DOMAIN, RESIDUES 23-271

GLVPRGSHMSGATEACLPAGQRKSGMNINFYQYSLKDSSTYSNAAYMAYGYASKTKLGSVGGQTDISIDYNIPCVSSSGT
FPCPQEDSYGNWGCKGMGACSNSQGIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFSFATVDDSAILSVGGSIAF
ECCAQEQPPITSTNFTINGIKPWDGSLPDNITGTVYMYAGYYYPLKVVYSNAVSWGTLPISVELPDGTTVSDNFEGYVYS
FDDDLSQSNCTIPDPSIH

0.5M NACL, 0.1 M BISTRIS-PROPANE PH7.5, 20% PEG4000

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D2-D3)

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE WITH MUTATION S227A IN COMPLEX WITH CALCIUM AND A1,2-MANNOBIOSE

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE WITH MUTATION D201T IN COMPLEX WITH CALCIUM AND GLUCOSE

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND A1,2-MANNOBIOSE

X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MANNOSE

D-MANNOSE (MAN): THESE RESIDUES CONSTITUTE MAN3D1 - MAN-A1, 2-MAN-A1,2-MAN

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY. RESIDUES 197 - 199 ARE NOT WELL DEFINED. RESIDUES 200 - 204 HAVE BEEN MODELLED IN TWO DISTINCT CONFORMATIONS.

CELL ADHESION, GREENBEARD, SOCIAL INTERACTION, PA14-DOMAIN, CARBOHYDRATE BINDING