Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor

Computational Design of a Chain-Specific Tetracycline Repressor Heterodimer.

TETRACYCLINE REPRESSOR PROTEIN CLASS B FROM TRANSPOSON TN10, TETRACYCLINE REPRESSOR PROTEIN CLASS D

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAVEILARHHDYSLPAAGESWQSFLR
NNAMSFRRALLRYRDGAKVHLGTRPDEKQYDTVETQLRFMTENGFSLRDGLYAISWVSHFTLGAVLEQQEHTAALTDRPA
APDENLPPLLREALQIMDSDDGEQAFLHGLESAIRGIEVQLTALLQIV

GSHMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAVEILARHHDYSLPAAGESWQS
FLRNNAMSFRRALLRYRDGAKVHLGTRPDEKQYDTVETQLRFMTENGFSLRDGLYAISAVSHATLGAVLEQQEHTAALTD
RPAAPDENLPPLLREALQIMDSDDGEQAFLHGLEWLIRGFEVQLTALLQIV

CRYSTAL STRUCTURE OF A DESIGNED HETERODIMERIC VARIANT T-A(I)B OF THE TETRACYCLINE REPRESSOR

TET REPRESSOR (CLASS D) IN COMPLEX WITH ISO-7-CHLORTETRACYCLINE

TETRACYCLINE CHELATED MG2+ -ION INITIATES HELIX UNWINDING FOR TET REPRESSOR INDUCTION

TETR (BD) VARIANT L17G WITH REVERSE PHENOTYPE

TET REPRESSOR, CLASS D IN COMPLEX WITH 9 -(N,N-DIMETHYLGLYCYLAMIDO)-6-DEMETHYL-6- DEOXY-TETRACYCLINE

TET REPRESSOR CLASS D IN COMPLEX WITH 7- CHLOR-2-CYANO-ISO-TETRACYCLINE

TET REPRESSOR CLASS D COMPLEXED WITH COBALT AND TETRACYCLINE

CRYSTAL STRUCTURE OF TETRACYCLINE REPRESSOR/ OPERATOR COMPLEX

CRYSTAL STRUCTURE OF A DESIGNED HOMODIMERIC VARIANT T-A(L)A(L) OF THE TETRACYCLINE REPRESSOR

CRYSTAL STRUCTURE OF TET REPRESSOR CLASS D WITH 4-EPI- TETRACYCLINE

TET REPRESSOR (CLASS D) IN COMPLEX WITH 7 -IODOTETRACYCLINE

MOL_ID: 1; MOLECULE: TETRACYCLINE REPRESSOR; CHAIN: NULL; SYNONYM: TET REPRESSOR, CLASS D ; ENGINEERED: YES

ENGINEERED RESIDUE IN CHAIN A, ALA 136 TO TRP ENGINEERED RESIDUE IN CHAIN B, PHE 140 TO ALA ENGINEERED RESIDUE IN CHAIN B, SER 192 TO TRP ENGINEERED RESIDUE IN CHAIN A, LEU 193 TO ALA ENGINEERED RESIDUE IN CHAIN A, PHE 197 TO ILE

ENGINEERED VARIANT, NAMELY CHIMERA OF P04483 AND P0ACT4 (
UNIPROT ACCESSION NUMBERS)
AUTHOR HAS MENTIONED THAT THE C-TERMINUS OF P0ACT4 HAS BEEN
TRUNCATED ARTIFICIALLY SINCE THE LATTER HAS 218 RESIDUES
IN TOTAL. FOR B CHAIN THERE IS AN ARTIFICIAL N-TERMINAL
EXTENSION PRESENT IN THE PROTEIN.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. ATOM RECORD CONTAINS SUM OF TLS AND RESIDUAL B FACTORS. ANISOU RECORD CONTAINS SUM OF TLS AND RESIDUAL U FACTORS. THE CRYSTAL STRUCTURE CORRESPONDS TO A HETERODIMER WITH CHAINS A AND B. BOTH CHAINS DIFFER AT ONLY 5 POSITIONS. THE DATA HAS BEEN EXPLAINED A BIMODAL DISORDER MODEL IN WHICH BOTH CHAINS ARE PRESENT WITH 0.5 OCCUPANCY IN THE ASYMMETRIC UNIT AT THE SAME TIME.

CHIMERA, TRANSCRIPTION, TRANSCRIPTION REGULATION, ANTIBIOTIC RESISTANCE