Structures of Sequential Open States in a Symmetrical Opening Transition of the Tolc Exit Duct.

MATURE PROTEIN WITH 43 C-TERMINAL RESIDUES REMOVED, RESIDUES 23-450

ENLMQVYQQARLSNPELRKSAADRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLDQTTQRFNVGLVAITDVQNARAQ
YDTVLANELTARNNLDNAVEQLRQITGNYYPELAALNVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQA
QDGHLPTLDLTASTGISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGASEQLESAH
RSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTSTIVDVLDATTTLYNAKQELANARYNYLINQLNI
KSALGTLNEQDLLALNNALSKPVSTNPE

50 MM TRIS PH 8.2, 50 MM NACL, 14% PEG2000, 0.075% N-DODECYL-BETA-D-MALTOSIDE, 0.3% N-OCTYL BETA-D-GLUCOPYRANOSIDE

2.1A X-RAY STRUCTURE OF TOLC: AN INTEGRAL OUTER MEMBRANEPROTEIN AND EFFLUX PUMP COMPONENT FROM ESCHERICHIA COLI

CRYSTAL STRUCTURE OF THE OPEN STATE OF TOLC OUTER MEMBRANE COMPONENT OF MUTLIDRUG EFFLUX PUMPS

TRYPTOPHAN SYNTHASE IN THE PRESENCE OF POTASSIUM AT ROOM TEMPERATURE

STRUCTURE OF TOLC IN COMPLEX WITH HEXAMMINECOBALT

CRYSTAL STRUCTURE OF THE OPEN STATE OF TOLC OUTER MEMBRANE COMPONENT OF MUTLIDRUG EFFLUX PUMPS RELATED STRUCTURES ARE WILD TYPE (PDBID 1EK9) STRUCTURE WITH R367S AND Y362F MUTATIONS (PDBID 2WM7)

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 13-STRANDED BARREL THIS IS REPRESENTED BY
A 14-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

ENGINEERED RESIDUE IN CHAIN A, ARG 389 TO SER ENGINEERED RESIDUE IN CHAIN B, ARG 389 TO SER ENGINEERED RESIDUE IN CHAIN C, ARG 389 TO SER
TRIETHYLENE GLYCOL (PGE): FRAGMENT OF PRECIPITANT
V TO L AT GENE POSITION 191 IN ISOLATE USED FOR STRUCTURE DETERMINATION - CORRESPONDS TO L169 IN THE MATURE FORM OF PROTEIN CRYSTALLIZED

GEOMETRIC REFINEMENT WAS CARRIED OUT USING CNS VERSION 1.1. B-FACTOR REFINEMENT, BULK SOLVENT MODELLING, AND FINAL R-FACTORS ARE FROM PHENIX. TRIETHYLENE GLYCOL PARAMETER FILE AND TOPOLOGY FILE WERE FROM HIC-UP DATABASE.

MEMBRANE PROTEIN, TYPE-I SECRETION, CELL OUTER MEMBRANE, TRANSPORT, DRUG-EFFLUX