Crystal structure of a double Ile-to-Met mutant of protein ORF34 from lactococcus phage p2
Structure and Function of Phage P2 Orf34(P2), a New Type of Single-Stranded DNA Binding Protein.
GTIITVTAQANEKNTRTVSTAKGDKKIISVPLFEKEKGSNVKVAYGSAF(MSE)PDFIQ(MSE)GDTVTVSGRVQAKESG EYVNYNFVFPTVEKVFITNDNSSQSQAKQDLFGGSEPIEVNSEDLPF
GTIITVTAQANEKNTRTVSTAKGDKKIISVPLFEKEKGSNVKVAYGSAFMPDFIQMGDTVTVSGRVQAKESGEYVNYNFV FPTVEKVFITNDNSSQSQAKQDLFGGSEPIEVNSEDLPF
PROTEIN AT 7 MG/ML IN 10 MM TRIS, 300 MM NACL, 1 MM TCEP, PH 8.0 WAS CRYSTALLIZED BY SITTING-DROP VAPOUR DIFFUSION AGAINST 0.1 M HEPES PH 7.2, 60% MME-PEG550
CRYSTAL STRUCTURE FROM A SINGLE-STRANDED DNA BINDING PROTEIN FROM THE LACTOCOCCAL PHAGE P2
ENGINEERED RESIDUE IN CHAIN A, LEU 62 TO MSE ENGINEERED RESIDUE IN CHAIN A, LEU 68 TO MSE
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
SSB, SINGLE-STRANDED DNA BINDING, DNA BINDING PROTEIN