IrisFP fluorescent protein in its red form, cis conformation

2-[(4Z)-4-[(4-hydroxyphenyl)methylidene]-2-[(E)-2-(1H-imidazol-4-yl)ethenyl]-5-oxidanylidene-imidazol-1-yl]ethanoic acid
RED CHROMOPHORE (HIS-TYR-GLY); {(2R)-4-(4-hydroxybenzyl)-2-[2-(1H-imidazol-4-yl)ethyl]-5-oxo-2,5-dihydro-1H-imidazol-1-yl}acetic acid

Structural Characterization of Irisfp, an Optical Highlighter Undergoing Multiple Photo-Induced Transformations.

Green to red photoconvertible GFP-like protein EosFP

(RC7)NRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKW
EPSTEKMYVRDGVLTGDITMALLLEGNAHYRCDSRTTYKAKEKGVKLPGYHLVDHCIEILSHDKDYNKVKLYEHAVAHSG
LPDNARR

XNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPST
EKMYVRDGVLTGDITMALLLEGNAHYRCDSRTTYKAKEKGVKLPGYHLVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
ARR

CRYSTALLIZATION CONDITIONS: AMMONIUM SULFATE, BICINE, PH 8.4, VAPOR DIFFUSION, TEMPERATURE 293K

IRISFP FLUORESCENT PROTEIN IN ITS GREEN FORM , CIS CONFORMATION

IRISFP FLUORESCENT PROTEIN IN ITS GREEN FORM , TRANS CONFORMATION

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY
A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY
A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "CA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY
A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "DA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY
A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

ENGINEERED RESIDUE IN CHAIN A, PHE 173 TO SER ENGINEERED RESIDUE IN CHAIN A, PHE 191 TO LEU ENGINEERED RESIDUE IN CHAIN B, PHE 173 TO SER ENGINEERED RESIDUE IN CHAIN B, PHE 191 TO LEU ENGINEERED RESIDUE IN CHAIN C, PHE 173 TO SER ENGINEERED RESIDUE IN CHAIN C, PHE 191 TO LEU ENGINEERED RESIDUE IN CHAIN D, PHE 173 TO SER ENGINEERED RESIDUE IN CHAIN D, PHE 191 TO LEU
RESIDUES HIS 62, TYR 63 AND GLY 64 AUTOCATALYTICALLY FORM THE CHROMOPHORE IEY LABELLED AS RESIDUE 64 IN THE COORDINATES.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

PHOTOACTIVATION, PHOTOCONVERSION, FLUORESCENT PROTEIN, OPTICAL HIGHLIGHTERS, MICROSPECTROPHOTOMETRY, EOSFP, PHOTOCHROMISM, PHOTOSWITCHING

HYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEP
STEKMYVRDGVLTGDITMALLLEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
DNARR