Crystal structure of N341AbsSHMT Gly external aldimine

Structural and Functional Studies of Bacillus Stearothermophilus Serine Hydroxymethyltransferase: The Role of Asn(341), Tyr(60) and Phe(351) in Tetrahydrofolate Binding.

SCHIFF LINKAGE BETWEEN GLY B 502 AND PLP B 501

MKYLPQQDPQVFAAIEQERKRQHAKIELIASENFVSRAVMEAQGSVLTNKYAEGYPGRRYYGGCEYVDIVEELARERAKQ
LFGAEHANVQPHSGAQANMAVYFTVLEHGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVAYGVDPETHVIDYDDVREKAR
LHRPKLIVAAASAYPRIIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPYAHFVTTTTHKTLRGPRGGMILCQE
QFAKQIDKAIFPGIQGGPLMHVIAAKAVAFGEALQDDFKAYAKRVVDNAKRLASALQNEGFTLVSGGTDNHLLLVDLRPQ
QLTGKTAEKVLDEVGITVNKATIPYDPESPFVTSGIRIGTAAVTTRGFGLEEMDEIAAIIGLVLKNVGSEQALEEARQRV
AALTD

50 % MPD, 0.1 M HEPES PH 7.5, 0.2 MM EDTA, 5 MM 2-MERCAPTOETHANOL, 10 MM GLY

K226M MUTANT OF SERINE HYDROXYMETHYLTRANSFERASE FROM B.STEAROTHERMOPHILUS, COMPLEX WITH SERINE

CRYSTAL STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASECOMPLEXED WITH GLYCINE AND 5-FORMYL TETRAHYDROFOLATE

CRYSTAL STRUCTURE OF S172ABSSHMT OBTAINED IN THE PRESENCE OF L-ALLO-THR

CRYSTAL STRUCTURE OF E53QBSSHMT INTERNAL ALDIMINE

K226M MUTANT OF SERINE HYDROXYMETHYLTRANSFERASE FROM B.STEAROTHERMOPHILUS

CRYSTAL STRUCTURE OF E53QBSSHMT OBTAINED IN THE PRESENCE OF L-ALLO-THREONINE

CRYSTAL STRUCTURE OF E53QBSSHMT WITH L-SERINE

K226Q MUTANT OF SERINE HYDROXYMETHYLTRANSFERASE FROM B.STEAROTHERMOPHILUS, COMPLEX WITH GLYCINE

CRYSTAL STRUCTURE OF S172ABSSHMT GLYCINE EXTERNAL ALDIMINE

CRYSTAL STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASECOMPLEXED WITH GLYCINE

CRYSTAL STRUCTURE OF S172ABSSHMT INTERNAL ALDIMINE

CRYSTAL STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASECOMPLEXED WITH SERINE

CRYSTAL STRUCTURE OF E53QBSSHMT WITH GLYCINE

CRYSTAL STRUCTURE OF S172ABSSHMT L-SERINE EXTERNAL ALDIMINE

CRYSTAL STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASE FROMB.STEAROTHERMOPHILUS

CRYSTAL STRUCTURE OF E53QBSSHMT OBTAINED IN THE PRESENCE OF GLYCINE AND 5-FOMYL TETRAHYDROFOLATE

CRYSTAL STRUCTURE OF N341ABSSHMT L-SER EXTERNAL ALDIMINE

CRYSTAL STRUCTURE OF Y60ABSSHMT INTERNAL ALDIMINE

CRYSTAL STRUCTURE OF Y60ABSSHMT CRYSTALLIZED IN THE PRESENCE OF L-ALLO-THR

CRYSTAL STRUCTURE OF F351GBSSHMT INTERNAL ALDIMINE

STRUCTURE OF N341ABSSHMT CRYSTALLIZED IN THE PRESENCE OF L-ALLO-THR

CRYSTAL STRUCTURE OF Y60ABSSHMT CRYSTALLIZED IN THE PRESENCE OF GLYCINE

CRYSTAL STRUCTURE OF Y60ABSSHMT L-SER EXTERNAL ALDIMINE

CRYSTAL STRUCTURE OF F351GBSSHMT IN COMPLEX WITH L-SER

CRYSTAL STRUCTURE OF F351GBSSHMT IN COMPLEX WITH L-ALLO-THR

CRYSTAL STRUCTURE OF N341ABSSHMT INTERNAL ALDIMINE

CRYSTAL STRUCTURE OF F351GBSSHMT IN COMPLEX WITH GLY AND FTHF

CRYSTAL STRUCTURE OF F351GBSSHMT IN COMPLEX WITH GLY

ENGINEERED RESIDUE IN CHAIN A, ASN 341 TO ALA

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

PLP-DEPENDENT ENZYMES, N341A, TRANSFERASE, FOLATE BINDING, PYRIDOXAL PHOSPHATE, ONE-CARBON METABOLISM