Structures of the Shigella Flexneri Type 3 Secretion System Protein Mxic Reveal Conformational Variability Amongst Homologues.

THE CONSTRUCT IS A DELETION OF THE FIRST 73 RESIDUES AND WAS METHYLATED CHEMICALLY FOLLOWING WALTER ET AL, STRUCTURE 14,1617-1622 (2006)

HSSGLVPRGSHMSQERILDGEEDEINH(MLY)IFDL(MLY)RTL(MLY)DNLPLDRDFIDRL(MLY)RYF(MLY)DPSDQ
VLALRELLNE(MLY)DLTAEQVELLT(MLY)IINEIISGSE(MLY)SVNAGINSAIQA(MLY)LFGN(MLY)M(MLY)LE
PQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFVEQSLIVDMDSE(MLY)PSCNAYEFGFVLS(MLY)LIAI
(MLY)MIRTSDVIFM(MLY)(MLY)LESSSLL(MLY)DGSLSAEQLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVV
YQTYLSSVNESPHDIF(MLY)SESEREIAINILRELVTSAY(MLY)(MLY)ELSR

HSSGLVPRGSHMSQERILDGEEDEINHKIFDLKRTLKDNLPLDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVEL
LTKIINEIISGSEKSVNAGINSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFVEQS
LIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAEQLLLTLLYIFQYPSESEQILTSVIE
VSRASHEDSVVYQTYLSSVNESPHDIFKSESEREIAINILRELVTSAYKKELSR

1.0 M SUCCINIC ACID, 0.1 M HEPES PH 7.0, 1% W/V PEGMME 2000

THE CONSTRUCT IS A DELETION OF RESIDUES 1-73 AND THEN HAS THE SEQUENCE HSSGLVPRGSHM FROM THE EXPRESSION VECTOR.

REFINED IN BUSTER-TNT VERSION 2.1.1 WITH RESTRAINTS BASED ON REFMAC5 THE LATTICE IS PSEUDOTETRAGONAL P 42 2 2 BUT REFINEMENT IN THIS SPACE GROUP WITH 1 MOL PER ASU DID NOT WORK WELL

SECRETION REGULATION, TRANSPORT PROTEIN, T3SS, VIRULENCE, TRANSPORT, TYPE THREE SECRETION SYSTEM