The crystal structure of alanine racemase from Bacillus anthracis (BA0252) with bound L-Ala-P

Oxford Protein Production Facility (OPPF)

(1S)-1-[((1E)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYLENE)AMINO]ETHYLPHOSPHONIC ACID

{1-[(3-HYDROXY-METHYL-5-PHOSPHONOOXY-METHYL-PYRIDIN-4-YLMETHYL)-AMINO]-ETHYL}-PHOSPHONIC ACID

Structures of an Alanine Racemase from Bacillus Anthracis (Ba0252) in the Presence and Absence of (R)-1-Aminoethylphosphonic Acid (L-Ala-P).

ALL LYSINES DIMETHYLATED EXCEPT K41 AND K260

GPMEEAPFYRDTWVEVDLDAIYNNVTHI(MLY)EFIPSDVEIFAVVKGNAYGHDYVPVA(MLY)IALEAGATRLAVAFLD
EALVLRRAGITAPILVLGPSPPRDINVAAENDVALTVFQ(MLY)EWVDEAI(MLY)LWDGSSTM(MLY)YHINFDSGMGR
IGIRER(MLY)EL(MLY)GFL(MLY)SLEGAPFLELEGVYTHFATADEVETSYFD(MLY)QYNTFLEQLSWL(MLY)EFG
VDP(MLY)FVHTANSAATLRFQGITFNAVRIGIAMYGLSPSVEIRPFLPF(MLY)LEPALSLHT(MLY)VAHIKQVI
(MLY)GDGISYNVTYRT(MLY)TEEWIATVAIGYADGWLRRLQGFEVLVNG(MLY)RVPIVGRVTMDQFMIHLPCEVPLG
T(MLY)VTLIGRQGDEYISATEVAEYSGTINYEIITTISFRVPRIFIRNG(MLY)VVEVINYLNDI

GPMEEAPFYRDTWVEVDLDAIYNNVTHIKEFIPSDVEIFAVVKGNAYGHDYVPVAKIALEAGATRLAVAFLDEALVLRRA
GITAPILVLGPSPPRDINVAAENDVALTVFQKEWVDEAIKLWDGSSTMKYHINFDSGMGRIGIRERKELKGFLKSLEGAP
FLELEGVYTHFATADEVETSYFDKQYNTFLEQLSWLKEFGVDPKFVHTANSAATLRFQGITFNAVRIGIAMYGLSPSVEI
RPFLPFKLEPALSLHTKVAHIKQVIKGDGISYNVTYRTKTEEWIATVAIGYADGWLRRLQGFEVLVNGKRVPIVGRVTMD
QFMIHLPCEVPLGTKVTLIGRQGDEYISATEVAEYSGTINYEIITTISFRVPRIFIRNGKVVEVINYLNDI

25% (W/V) POLYETHYLENE GLYCOL 3350, 0.1M BIS-TRIS PH6.5, SOAKED IN 10MM L-ALA-P FOR 45 MINUTES, CRYOPROTECTED IN 25% GLYCEROL

THE CRYSTAL STRUCTURE OF ALANINE RACEMASE FROM BACILLUS ANTHRACIS (BA0252)

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AB", "BB" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN
ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW,
TWO SHEETS ARE DEFINED.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

PYRIDOXAL 5'-PHOSPHATE, PEPTIDOGLYCAN SYNTHESIS, PLP, OPPF, L-ALANINE, ISOMERASE, D-ALANINE, PYRIDOXAL PHOSPHATE, STRUCTURAL GENOMICS, ALANINE RACEMASE, SPORE GERMINATION, OXFORD PROTEIN PRODUCTION FACILITY (OPPF), STRUCTURAL PROTEOMICS IN EUROPE (SPINE)