The crystal structure of the human G-protein subunit alpha (GNAI3) in complex with an engineered regulator of G-protein signaling type 2 domain (RGS2)

Structural Determinants of G-Protein Alpha Subunit Selectivity by Regulator of G-Protein Signaling 2(Rgs2).

GUANINE NUCLEOTIDE-BINDING PROTEIN G(K) SUBUNIT ALPHA

G(I) ALPHA-3, GUANINE NUCLEOTIDE-BINDING PROTEIN G K SUBUNIT ALPHA G I ALPHA-3

G0/G1 SWITCH REGULATORY PROTEIN 8, RGS2, CELL GROWTH-INHIBITING GENE 31 PROTEIN

SMTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSI
IAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDR
ISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMN
RMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYT
HFTCATDTKNVQFVFDAVTDVIIKNNLKES

SNAKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFSEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKE
INIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENDSYPRFLKSEFYQDLCKKPQITTEPH

0.1 M HEPES PH 7.5, 2 M AMMONIUM SULPHATE

CRYSTAL STRUCTURE OF THE HETERODIMERIC COMPLEX OF HUMANRGS10 AND ACTIVATED GI ALPHA 3

STRUCTURE OF THE REGULATOR OF G-PROTEIN SIGNALING DOMAIN OFRGS2

ENGINEERED RESIDUE IN CHAIN B, CYS 106 TO SER ENGINEERED RESIDUE IN CHAIN B, ASN 184 TO ASP ENGINEERED RESIDUE IN CHAIN B, GLU 191 TO LYS

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

GTP HYDROLYSIS, ADP-RIBOSYLATION, NUCLEOTIDE-BINDING, LIPOPROTEIN, GTP-BINDING, PHOSPHOPROTEIN, SIGNAL TRANSDUCTION INHIBITOR, GUANINE NUCLEOTIDE BINDING PROTEIN, TRANSMEMBRANE SIGNALING, G-PROTEIN COUPLED RECEPTOR, PALMITATE, MYRISTATE, TRANSDUCER, CELL CYCLE