Crystal structure of RseB: a sensor for periplasmic stress response in E. coli

The Structure of Rseb: A Sensor in Periplasmic Stress Response of E. Coli.

MATPASGALLQQMNLASQSLNYELSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFT
LNGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWMDTESKLPMRVDLLDRDGETLE
QFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKAKFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSF
SVNVNRATPSSTDQMLRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQHHHHHH

PROTEIN WAS CRYSTALLIZED FROM 0.2 M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1 M TRIS-HCL PH 8.5, 25%(W/V) POLYETHYLENE GLYCOL 3350 AND 10 MM L-CYSTEINE

HELIX
DETERMINATION METHOD: AUTHOR PROVIDED.

SEEMS TO MODULATE THE ACTIVITY OF RPOE (SIGMA-E)
RESIDUE 22 IS A METHIONINE WHICH WAS CLONED THERE TO HAVE A START CODON. RESIDUES 319-324 DO NAT MATCH THE P0AFX9 AS IT AS A HIS-TAG, NEEDED FOR PURIFICATION OF THE PROTEIN

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

SENSOR FOR PERIPLASMIC STRESS, REGULATOR, REGULATORY PROTEIN, LIPOPROTEIN BINDING