Structure of isoniazid (INH) bound to cytochrome c peroxidase mutant N184R Y36A

ISONIAZID; TUBAZID; RIMITSID; ISONICOTINYLHYDRAZINE; LANIZID; NYDRAZID

The Tuberculosis Prodrug Isoniazid Bound to Activating Peroxidases.

MKTLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNAIGYGPVLVRLAWHTSGTWDKHDNTGGSYGGTYRFKKEFNDP
SNAGLQNGFKFLEPIHKEFPWISSGDLFSLGGVTAVQEMQGPKIPWRCGRVDTPEDTTPDNGRLPDADKDADYVRTFFQR
LNMNDREVVALMGAHALGKTHLKRSGYEGPWGAANNVFTNEFYLNLLNENWKLEKNDANNEQWDSKSGYMMLPTDYSLIQ
DPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQGL

MICRODIALYSIS INTO 50MM POTASSIUM PHOSPHATE, PH 6.0 CONTAINING 30% V/V MPD. ISONIAZID WAS DISSOLVED INTO THE MOTHER LIQUOR (300MM) AND SOAKED INTO THE CRYSTAL.

PROBING THE STRENGTH AND CHARACTER OF AN ASP-HIS-X HYDROGEN BOND BY INTRODUCING BURIED CHARGES

SPECIFICITY OF LIGAND BINDING IN A BURIED POLAR CAVITY OF CYTOCHROME C PEROXIDASE

VARIATION IN THE STRENGTH OF A CH TO O HYDROGEN BOND IN ANARTIFICIAL PROTEIN CAVITY (2,3,4-TRIMETHYL-1,3-THIAZOLE)

VARIATION IN THE STRENGTH OF A CH TO O HYDROGEN BOND IN ANARTIFICIAL PROTEIN CAVITY (3,4,5-TRIMETHYLTHIAZOLE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (3-METHYLTHIAZOLE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (3,4-DIMETHYLTHIAZOLE )

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (ANILINE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (3-AMINOPYRIDINE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (4-AMINOPYRIDINE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (2-AMINO-4- METHYLTHIAZOLE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (1-VINYLIMIDAZOLE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (INDOLINE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (IMIDAZO[1,2-A] PYRIDINE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (2-AMINO-5- METHYLTHIAZOLE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (2-AMINOPYRIDINE)

VARIATION IN THE STRENGTH OF A CH TO O HYDROGEN BOND IN ANARTIFICIAL PROTEIN CAVITY (2-ETHYLIMIDAZOLE)

SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (IMIDAZOLE)

VARIATION IN THE STRENGTH OF A CH TO O HYDROGEN BOND IN ANARTIFICIAL PROTEIN CAVITY (1-METHYLIMIDAZOLE)

SPECIFICITY OF LIGAND BINDING IN A POLAR CAVITY OF CYTOCHROME C PEROXIDASE (2- METHYLIMIDAZOLE)

INTRODUCTION OF NOVEL SUBSTRATE OXIDATION INTO CYTOCHROME C PEROXIDASE BY CAVITY COMPLEMENTATION: OXIDATION OF 2-AMINOTHIAZOLE AND COVALENT MODIFICATION OF THE ENZYME (2- AMINOTHIAZOLE)

INTERACTION BETWEEN PROXIMAL AND DISTALS REGIONS OF CYTOCHROME C PEROXIDASE

EFFECT OF UNNATURAL HEME SUBSTITUTION ON KINETICS OF ELECTRON TRANSFER IN CYTOCHROME C PEROXIDASE

MANGANESE BINDING MUTANT IN CYTOCHROME C PEROXIDASE

CYTOCHROME C PEROXIDASE (CCP-MKT) WILD TYPE

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH ASP 235 REPLACED BY GLU (D235E)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH ASP 235 REPLACED BY ALA (D235A)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH HIS 175 REPLACED BY GLY (H175G)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH HIS 175 REPLACED BY GLY (H175G) COMPLEXED WITH IMIDAZOLE

HOW FLEXIBLE ARE PROTEINS? TRAPPING OF A FLEXIBLE LOOP

CONFORMER SELECTION BY LIGAND BINDING OBSERVED WITH PROTEINCRYSTALLOGRAPHY

ALTERING SUBSTRATE SPECIFICITY OF CYTOCHROME C PEROXIDASE TOWARDS A SMALL MOLECULAR SUBSTRATE PEROXIDASE BY SUBSTITUTING TYROSINE FOR PHE 202

CYTOCHROME C PEROXIDASE (RECOMBINANT YEAST, CCP-MKT) MUTANT WITH TRP 191 REPLACED BY GLY (W191G) COMPLEXED WITH 1,2- DIMETHYLIMADAZOLE

CYTOCHROME C PEROXIDASE (RECOMBINANT YEAST, CCP-MKT) MUTANT WITH TRP 191 REPLACED BY GLY (W191G)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH INITIAL MET, LYS, THR AND WITH TRP 191 REPLACED BY GLY (INS(M1,K2,T3),W191G ) AND SOAKED IN 40 MILLIMOLAR POTASSIUM (K +)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH INITIAL MET, LYS, THR AND WITH TRP 191 REPLACED BY GLY AND ASP 235 REPLACED BY ASN (INS(M1,K2,T3),W191G,D235N) AND SOAKED IN 40 MILLIMOLAR POTASSIUM (K+)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND TRP 191 REPLACED BY GLY (MI,W191G) COMPLEXED WITH AN AMMONIUM ION (NH4+)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND TRP 191 REPLACED BY GLY (MI,W191G) COMPLEXED WITH A POTASSIUM ION (K+)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND TRP 191 REPLACED BY GLY (MI,W191G) COMPLEXED WITH A TRIS (+) ION

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND TRP 191 REPLACED BY GLN (MI,W191Q)

MOL_ID: 1; MOLECULE: CYTOCHROME C PEROXIDASE ; CHAIN: NULL; EC: 1.11.1.5; ENGINEERED : YES; MUTATION: INS(MET ILE AT N- TERMINUS), C128A, A193C

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND TRP 191 REPLACED BY PHE (MI,W191F) COMPLEXED WITH DIOXYGEN

CRYSTAL STRUCTURE OF R48A MUTANT OF CYTOCHROME C PEROXIDASE

CRYSTAL STRUCTURE OF R48A MUTANT OF CYTOCHROME C PEROXIDASE WITH N- HYDROXYGUANIDINE BOUND

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX, PH 6, 100K

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX, WITH PHOSPHATE BOUND, PH 6, 100K

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX AT PH 5, ROOM TEMPERATURE .

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX AT PH 6, ROOM TEMPERATURE .

CYTOCHROME C PEROXIDASE H175G MUTANT, IMIDAZOLE COMPLEX AT PH 7, ROOM TEMPERATURE .

LAUE DIFFRACTION STUDY ON THE STRUCTURE OF CYTOCHROME C PEROXIDASE COMPOUND I

STABILIZATION OF THE ENGINEERED CATION-BINDING LOOP INCYTOCHROME C PEROXIDASE (CCP)

CRYSTAL STRUCTURE OF A PROXIMAL DOMAIN POTASSIUM BINDINGVARIANT OF CYTOCHROME C PEROXIDASE

CRYSTAL STRUCTURE OF MESOPONE CYTOCHROME C PEROXIDASE(MPCCP)

ENGINEERING CALCIUM-BINDING SITE INTO CYTOCHROME CPEROXIDASE (CCP)

CRYSTAL STRUCTURE OF CYTOCHROME C PEROXIDASE WITH APROPOSED ELECTRON TRANSFER PATHWAY EXCISED TO FORM ALIGAND BINDING CHANNEL.

CRYSTAL STRUCTURE OF A MUTANT CYTOCHROME C PEROXIDASESHOWING A NOVEL TRP-TYR COVALENT CROSS-LINK

CRYSTAL STRUCTURE OF THE MUTANT VARIANT OF CYTOCHROME CPEROXIDASE IN THE 'OPEN' UNCROSS- LINKED FORM

CRYSTAL STRUCTURE OF A MUTANT VARIANT OF CYTOCHROME CPEROXIDASE (PLATE LIKE CRYSTALS)

CRYSTAL STRUCTURE OF A MUTANT VARIANT OF CYTOCHROME CPEROXIDASE WITH ZN(II)-(20-OXO- PROTOPORPHYRIN IX)

CYTOCHROME C PEROXIDASE W191G FROM SACCHAROMYCES CEREVISIAE

STRUCTURE OF A CYTOCHROME C PEROXIDASE- CYTOCHROME C SITESPECIFIC CROSS-LINK

STRUCTURE OF REDUCED-NO ADDUCT OF MESOPONE CYTOCHROME CPEROXIDASE

CYRSTAL STRUCTURE OF CYTOCHROME C PEROXIDASE MUTANT: CCPK2M2

CYRSTAL STRUCTURE OF CYTOCHROME C PEROXIDASE MUTANT: CCPK2M3

ELECTRON TRANSFER COMPLEX BETWEEN CYTOCHROME C ANDCYTOCHROME C PEROXIDASE

ELECTRON TRANSFER COMPLEX BETWEEN HORSE HEART CYTOCHROME CAND ZINC-PORPHYRIN SUBSTITUTED CYTOCHROME C PEROXIDASE

THE 1.13 ANGSTROM STRUCTURE OF IRON-FREE CYTOCHROME CPEROXIDASE

HIGH-RESOLUTION CRYSTAL STRUCTURE OF NATIVE ( RESTING)CYTOCHROME C PEROXIDASE (CCP)

HIGH-RESOLUTION CRYSTAL STRUCTURE OF COMPOUND IINTERMEDIATE OF CYTOCHROME C PEROXIDASE ( CCP)

CRYSTAL STRUCTURE OF THE PROTEIN-PROTEIN COMPLEX BETWEENF82I CYTOCHROME C AND CYTOCHROME C PEROXIDASE

CRYSTAL STRUCTURE OF THE PROTEIN-PROTEIN COMPLEX BETWEENF82S CYTOCHROME C AND CYTOCHROME C PEROXIDASE

CRYSTAL STRUCTURE OF THE PROTEIN-PROTEIN COMPLEX BETWEENF82W CYTOCHROME C AND CYTOCHROME C PEROXIDASE

CRYSTAL STRUCTURE OF THE PROTEIN-PROTEIN COMPLEX BETWEENF82Y CYTOCHROME C AND CYTOCHROME C PEROXIDASE

SOLVENT ISOTOPE EFFECTS ON INTERFACIAL PROTEIN ELECTRONTRANSFER BETWEEN CYTOCHROME C AND CYTOCHROME C PEROXIDASE

YEAST CYTOCHROME C PEROXIDASE MUTANT WITH ASP 235 REPLACED BY ASN (D235N)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND MET 230 REPLACED BY ILE (MI,M230I)

CYTOCHROME C PEROXIDASE (FERROCYTOCHROME C ( COLON) H2O2 REDUCTASE)

SOLUTION STRUCTURE OF THE COMPLEX BETWEEN YEAST ISO-1-CYTOCHROME C AND YEAST CYTOCHROME C PEROXIDASE

YEAST CYTOCHROME C PEROXIDASE (CCP) COMPLEX WITH HORSE HEART CYTOCHROME C

YEAST CYTOCHROME C PEROXIDASE (CCP) COMPLEX WITH YEAST ISO-1-CYTOCHROME C

STRUCTURE OF CYTOCHROME C PEROXIDASE MUTANT N184R Y36A

YEAST CYTOCHROME C PEROXIDASE MUTANT WITH TRP 191 REPLACED BY PHE (W191F)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH MET-LYS-THR INSERTED AT THE N- TERMINUS, THR 52 REPLACED BY ILE, ALA 147 REPLACED BY TYR, ASP 152 REPLACED BY GLY (INS(M1,K2,T3),T52I,A147Y,D152G)

YEAST CYTOCHROME C PEROXIDASE MUTANT WITH TRP 51 REPLACED BY PHE (W51F)

CYTOCHROME C PEROXIDASE (CCP-MKT) MUTANT WITH MET-LYS-THR INSERTED AT THE N- TERMINUS, THR 53 REPLACED BY ILE, ALA 147 REPLACED BY MET, ASP 152 REPLACED BY GLY (INS(M1,K2,T3),T53I,A147M,D152G)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND HIS 52 REPLACED BY LEU (MI,H52L)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND ARG 48 REPLACED BY LYS (MI,R48K)

CYTOCHROME C PEROXIDASE MUTANT WITH MET ILE ADDED AT N-TERMINUS AND ARG 48 REPLACED BY LEU (MI,R48L)

ENGINEERED RESIDUE IN CHAIN A, TYR 106 TO ALA ENGINEERED RESIDUE IN CHAIN A, ASN 251 TO ARG

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

CYTOCHROME C PEROXIDASE, TRANSIT PEPTIDE, ORGANIC RADICAL, HYDROGEN PEROXIDE, INH, CCP, IRON, HEME, ISONIAZID, PEROXIDASE, MITOCHONDRION, METAL-BINDING, OXIDOREDUCTASE