Crystal structure of human P450 3A4 in complex with ketoconazole

Structural Basis for Ligand Promiscuity in Cytochrome P450 3A4

QUININE 3-MONOOXYGENASE, CYPIIIA4, NIFEDIPINE OXIDASE, TAUROCHENODEOXYCHOLATE 6-ALPHA- HYDROXYLASE, NF-25, P450-PCN1

MAYGTHSHGLFKKLGIPGPTPLPFLGNILSYHKGFCMFDMECHKKYGKVWGFYDGQQPVLAITDPDMIKTVLVKECYSVF
TNRRPFGPVGFMKSAISIAEDEEWKRLRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKDVFGAYSMD
VITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLRKSVKRMKESRLE
DTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNK
APPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFSKK
NKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSG
AHHHH

HANGING DROP CRYSTALLISATION USING 1:1 RATIO OF PROTEIN AT 20-27 MG/ML IN A SOLUTION CONTAINING 50 MM POTASSIUM PHOSPHATE PH 7.2, 0.2 MM POTASSIUM CHLORIDE, 20% (V/V) GLYCEROL, 2 MM DITHIOTHREITOL, 1MM EDTA

CRYSTAL STRUCTURE OF HUMAN MICROSOMAL P450 3A4

CRYSTAL STRUCTURE OF HUMAN CYTOCHROME P450 3A4

CRYSTAL STRUCTURE OF HUMAN P450 3A4 IN COMPLEX WITH ERYTHROMYCIN

THE SEQUENCE CONFLICTS CAN BE DERIVED TO A SEQUENCING ERROR IN THE ORIGINAL WORK DESCRIBING THE PROTEIN SEQUENCE, REFERENCES ARE GIVEN IN THE UNIPROT ENTRY

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

METAL-BINDING, TRANSMEMBRANE, OXIDOREDUCTASE, ENDOPLASMIC RETICULUM, DRUG METABOLIZING ENZYME, KETOCONAZOL, POLYMORPHISM, MONOOXYGENASE, P450, NADP, IRON, HEME, CYP3A4, MEMBRANE, MICROSOME