Refined solution structure of Schizosaccharomyces pombe Sin1 CRIM domain
Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit.
Stress-activated map kinase-interacting protein 1
GPGHMGSVSNAKAPTSALRALLEHKENSSQNGPLAENFATFSGHAESNALRLNIYFPSSESPSKPLFVELRKNVLVSEAI GYILLQYVNQQLVPPIEDEAQNPNYWNLRIVEDDGELDEDFPALDRVGPLSKFGFDAFALVKATPAQIKENQAAYPFKSK
0.5 mM [U-100% 15N] Sin1CRIM-1, 50 mM potassium phosphate-2, 50 mM potassium chloride-3, 90 % H2O-4, 10 % D2O-5, 1 mM DTT-6, 90% H2O/10% D2O
0.5 mM [U-100% 13C; U-100% 15N] Sin1CRIM-7, 50 mM potassium phosphate-8, 50 mM potassium chloride-9, 1 mM DTT-10, 90 % H2O-11, 10 % D2O-12, 90% H2O/10% D2O
0.5 mM Sin1CRIM-13, 50 mM potassium phosphate-14, 50 mM potassium chloride-15, 90 % H2O-16, 10 % D2O-17, 1 mM DTT-18, 90% H2O/10% D2O
0.5 mM [U-100% 15N] Sin1CRIM-19, 50 mM potassium phosphate-20, 50 mM potassium chloride-21, 90 % H2O-22, 10 % D2O-23, 1 mM DTT-24, 15 mg/mL Pf1 phage-25, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore
Sin1, CRIM domain, TORC2, paramagnetic relaxation enhancement, CELL CYCLE
SVSNAKAPTSALRALLEHKENSSQNGPLAENFATFSGHAESNALRLNIYFPSSESPSKPLFVELRKNVLVSEAIGYILLQ YVNQQLVPPIEDEAQNPNYWNLRIVEDDGELDEDFPALDRVGPLSKFGFDAFALVKATPAQIKENQAAYPFKSK