Chemical Shift Assignments for MIP and MDM2 in bound state
Structural Basis for Inhibition of the MDM2:p53 Interaction by an Optimized MDM2-Binding Peptide Selected with mRNA Display
Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
MPRFWEYWLRLMEGGGENLYFQGMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIM TKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMMASMTGGQQMG
Protein expresses as HAT-GB1-(Thrombin cleavage site)-MIP-(TEV cleavage site)-MDM2-T7tag fusion.
AMBER12 using the AMBER 2003 force field and a generalized Born model
650 uM [U-100% 13C; U-100% 15N] MIP-MDM2 fusion-1, 20 mM TRIS-2, 300 mM sodium chloride-3, 95% H2O/5% D2O
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Ubiquitin Ligase, PEPTIDE BINDING PROTEIN
MSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDL FGVPSFSVKEHRKIYTM