Solution Structure of the Q41N Variant of Ubiquitin as a Model for the Alternatively Folded N2 State of Ubiquitin
High-energy state model mutant (Q41N) for ubiquitin
torsion angle dynamics, Energy minimization
0.5 mM [U-15N] protein-1, 20 mM [U-99% 2H] TRIS-2, 1 mM DSS-3, 250 mM sodium chloride-4, 6 mg/mL Pf1 bacteriopharge-5, 93% H2O/7% D2O
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman
ubiquitin, Q41N, high energy, N2, PROTEIN BINDING