Crystal Structure of Lhx3 LIM domains 1 and 2 with the binding domain of Isl1

Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes.

THERE ARE SEVERAL CHIRALITY ERRORS IN CHAIN A and B.

Fusion of Lhx3 residues 28-153 to Isl1 residues 262-291 via a glycine rich linker with sequence GGSGGHMGSGG
Fusion of LIM/homeobox protein Lhx3, linker, Insulin gene enhancer protein ISL-1

LIM homeobox protein 3, Homeobox protein LIM-3, Homeobox protein P-LIM, Islet-1

GSTPEIPMCAGCDQHILDRFILKALDRHWHSKCLKCSDCHVPLAERCFSRGESVYCKDDFFKRFGTKCAACQLGIPPTQV
VRRAQDFVYHLHCFACVVCKRQLATGDEFYLMEDSRLVCKADYETAKQGGSGGSGGSGGGTPMVAASPERHDGGLQANPV
EVQSYQPPW

1.0M tri-sodium citrate, 0.1M Tris, HCl pH 7.25, Vapour diffusion, Hanging drop, temperature 298K

FUSION OF LHX3 RESIDUES 28-153 TO ISL1 RESIDUES 262-291 VIA A GLYCINE RICH LINKER WITH SEQUENCE GGSGGSGGSGG. RESIDUE NUMBERS 165-261 ARE SIMPLY SKIPPED.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

protein-protein complex, LIM domain, Zn finger, Activator, DNA-binding, Homeobox, Metal-binding, Nucleus, Transcription, Transcription regulation, METAL BINDING PROTEIN

TPEIPMCAGCDQHILDRFILKALDRHWHSKCLKCSDCHVPLAERCFSRGESVYCKDDFFKRFGTKCAACQLGIPPTQVVR
RAQDFVYHLHCFACVVCKRQLATGDEFYLMEDSRLVCKADYETAKQ