Crystal Structure of PtlH with Fe/oxalylglycine bound

Crystal Structure of the Non-heme Iron Dioxygenase PtlH in Pentalenolactone Biosynthesis.

1-deoxypentalenic acid 11-beta hydroxylase; Fe(II)/alpha-ketoglutarate dependent hydroxylase

GSHMTNVTGDYTDCTPLLGDRAALDSFYEEHGYLFLRNVLDRDLVKTVAEQMREGLVALGAADPHATLEELTIDSFESVD
EVAMHDYVKYDAFWNNPSTIKVFEQVFGEPVFVFLSTTIRYYPSQAGSEEPSFHYLTPFHQDGFYIGPNQDFRTFWIPLI
RTTRESGGVALADGSHRRGKRDHVLNESFRRFGHPVRGIPPTEVSEDEHLLHSPMEPGDILLFHAHMCHKSIPNLSKDPR
LMRMSMDTRVQPAKSHRGFNAMTPWTESAKDASKGIMAKITGTPTDVE

100mM HEPES, 200mM MgCl2, 20% PEG 3350, pH 7.5, sitting drop, temperature 282K

Crystal structure of PtlH with iron/alpha ketoglutarate and ent -1-deoxypentalenic acid bound

Crystal structure of PtlH with iron/alpha ketoglutarate bound

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

double stranded barrel helix, dioxygenase, OXIDOREDUCTASE

MTNVTGDYTDCTPLLGDRAALDSFYEEHGYLFLRNVLDRDLVKTVAEQMREGLVALGAADPHATLEELTIDSFESVDEVA
MHDYVKYDAFWNNPSTIKVFEQVFGEPVFVFLSTTIRYYPSQAGSEEPSFHYLTPFHQDGFYIGPNQDFRTFWIPLIRTT
RESGGVALADGSHRRGKRDHVLNESFRRFGHPVRGIPPTEVSEDEHLLHSPMEPGDILLFHAHMCHKSIPNLSKDPRLMR
MSMDTRVQPAKSHRGFNAMTPWTESA