Wild type human ferrochelatase crystallized with ammonium sulfate

A pi-Helix Switch Selective for Porphyrin Deprotonation and Product Release in Human Ferrochelatase.

RKPKTGILMLNMGGPETLGDVHDFLLRLFLDRDLMTLPIQNKLAPFIAKRRTPKIQEQYRRIGGGSPIKIWTSKQGEGMV
KLLDELSPNTAPHKYYIGFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNQVGRKPTMKWSTI
DRWPTHHLLIQCFADHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQEVSATVQKVMERLEYCNPYRLVWQSK
VGPMPWLGPQTDESIKGLCERGRKNILLVPIAFTSDHIETLYELDIEYSQVLAKECGVENIRRAESLNGNPLFSKALADL
VHSHIQSNELCSKQLTLSCPLCVNPVCRETKSFFTSQQL

0.05M ammonium sulfate, 0.05M Bis-Tris, pH 6.5 and 40% (v/v) pentaerythritol ethoxylate (15/4 EO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 291K

Heme synthesis, Ferrochelatase, Protoporphyrin IX, LYASE