Crystal structure of Medicago truncatula UGT85H2- Insights into the structural basis of a multifunctional (Iso) flavonoid glycosyltransferase

Crystal Structure of Medicago truncatula UGT85H2 - Insights into the Structural Basis of a Multifunctional (Iso)flavonoid Glycosyltransferase.

MGNFANRKPHVVMIPYPVQGHINPLFKLAKLLHLRGFHITFVNTEYNHKRLLKSRGPKAFDGFTDFNFESIPDGLTPMEG
DGDVSQDVPTLCQSVRKNFLKPYCELLTRLNHSTNVPPVTCLVSDCCMSFTIQAAEEFELPNVLYFSSSACSLLNVMHFR
SFVERGIIPFKDESYLTNGCLETKVDWIPGLKNFRLKDIVDFIRTTNPNDIMLEFFIEVADRVNKDTTILLNTFNELESD
VINALSSTIPSIYPIGPLPSLLKQTPQIHQLDSLDSNLWKEDTECLDWLESKEPGSVVYVNFGSTTVMTPEQLLEFAWGL
ANCKKSFLWIIRPDLVIGGSVIFSSEFTNEIADRGLIASWCPQDKVLNHPSIGGFLTHCGWNSTTESICAGVPMLCWPFF
ADQPTDCRFICNEWEIGMEIDTNVKREELAKLINEVIAGDKGKKMKQKAMELKKKAEENTRPGGCSYMNLNKVIKDVLLK
QN

20% PEG3350, 0.2M magnesium formate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

NPPSFA, National Project on Protein Structural and Functional Analyses

SEQUENCE
Authors state that residue at position 305 is indeed THR.

glycosylation, isoflavonoid, uridine diphosphate glycosyltransferase, Structural Genomics, TRANSFERASE

MGNFANRKPHVVMIPYPVQGHINPLFKLAKLLHLRGFHITFVNTEYNHKRLLKSRGPKAFDGFTDFNFESIPDGLTPMEG
DGDVSQDVPTLCQSVRKNFLKPYCELLTRLNHSTNVPPVTCLVSDCCMSFTIQAAEEFELPNVLYFSSSACSLLNVMHFR
SFVERGIIPFKDESYLTNGCLETKVDWIPGLKNFRLKDIVDFIRTTNPNDIMLEFFIEVADRVNKDTTILLNTFNELESD
VINALSSTIPSIYPIGPLPSLLKQTPQIHQLDSLDSNLWKEDTECLDWLESKEPGSVVYVNFGSITVMTPEQLLEFAWGL
ANCKKSFLWIIRPDLVIGGSVIFSSEFTNEIADRGLIASWCPQDKVLNHPSIGGFLTHCGWNSTTESICAGVPMLCWPFF
ADQPTDCRFICNEWEIGMEIDTNVKREELAKLINEVIAGDKGKKMKQKAMELKKKAEENTRPGGCSYMNLNKVIKDVLLK
QN