Solution Structure of an rRNA Substrate Bound to the Pseudouridylation Pocket of a Box H/ACA snoRNA
RNA hairpin containing the pseudouridylation pocket
Synthesized by in vitro transcription using T7 RNA polymerase from linearized plasmid DNA. This sequence naturally occurs in humans.
5 mM cacodylate, 50 mM NaCl and 0.1 mM EDTA.
torsion angle dynamics,simulated annealing
Uniform labeling with 15N: U-15N Uniform labeling with 13C: U-13C
box H/ACA snoRNA, pseudouridylation, rRNA, RNA