The SoxYZ complex of Paracoccus pantotrophus

The SoxYZ Complex Carries Sulfur Cycle Intermediates on a Peptide Swinging Arm.

ADDAKPRVKVPSSAKAGETVTVKALISHK(MSE)ESGQRKDADGKLIPRSIINRFTCELNGVNVVDVAIDPAVSTNPYFE
FDAKVDAAGEFKFTWYDDDGSVYEDVKPIAVA

ADDAKPRVKVPSSAKAGETVTVKALISHKMESGQRKDADGKLIPRSIINRFTCELNGVNVVDVAIDPAVSTNPYFEFDAK
VDAAGEFKFTWYDDDGSVYEDVKPIAVA

HGSSTVDELTAAFTGGAATGEGGLTLTAPEIAENGNTVPIEVKAPGAVAI(MSE)LLAAGNPEPAVATFNFGPAAADQRA
ATRIRLAQTQDVIALAK(MSE)ADGSVVKAQTTVKVTIGG(CME)GG

HGSSTVDELTAAFTGGAATGEGGLTLTAPEIAENGNTVPIEVKAPGAVAIMLLAAGNPEPAVATFNFGPAAADQRAATRI
RLAQTQDVIALAKMADGSVVKAQTTVKVTIGGCGG

2 microl of protein at 10 mg/ml in 10 mM Tris-HCl pH 8.0, 5 mM beta-mercaptoethanol and 200 mM NaCl mixed with 2 microl of 28-30 % (w/v) PEG 3350, 100 mM sodium acetate pH 4.75, 200 mM NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The biological assembly is a SoxYZ heterodimer. The crystallographic asymmetric unit contains two copies of this biological assembly.

IMMUNOGLOBULIN-LIKE BETA-SANDWICH FOLD, Transport Protein

STVDELTAAFTGGAATGEGGLTLTAPEIAENGNTVPIEVKAPGAVAIMLLAAGNPEPAVATFNFGPAAADQRAATRIRLA
QTQDVIALAKMADGSVVKAQTTVKVTIGGCGG