Structure of the W47A/W242A Mutant of Bacterial Phosphatidylinositol-Specific Phospholipase C

Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C.

1-phosphatidylinositol phosphodiesterase

Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC

ASSVNELENWSKWMQPIPDNIPLARISIPGTHDSGTFKLQNPIKQVAGMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVL
HHGPLYLYVTLHEFINEAKQFLKDNPSETIIMSLKKEYEDMKGAEGSFSSTFEKNYFVDPIFLKTEGNIKLGDARGKIVL
LKRYSGSNESGGYNNFYWPDNETFTTTVNQNVNVTVQDKYKVNYDEKVKSIKDTMDETMNNSEDLNHLYINFTSLSSGGT
AANSPYYYASYINPEIANDIKQKNPTRVGWVIQDYINEKWSPLLYQEVIRANKSLI

15% 2-methyl-2,4-pentanediol, 16% PEG400, pH 5.4, vapor diffusion, temperature 290K

The biological unit is a dimer. There are two monomers in the asymmetric unit. The two monomers follow a pseudo 2-fold symmetry

phosphatidylinositol-specific phospholipase C, PI-PLC, Dimer, interfacially impaired, membrane binding, TIM barrel, Lyase

ASSVNELENWSKWMQPIPDNIPLARISIPGTHDSGTFKLQNPIKQVWGMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVL
HHGPLYLYVTLHEFINEAKQFLKDNPSETIIMSLKKEYEDMKGAEGSFSSTFEKNYFVDPIFLKTEGNIKLGDARGKIVL
LKRYSGSNESGGYNNFYWPDNETFTTTVNQNVNVTVQDKYKVNYDEKVKSIKDTMDETMNNSEDLNHLYINFTSLSSGGT
AWNSPYYYASYINPEIANDIKQKNPTRVGWVIQDYINEKWSPLLYQEVIRANKSLI