E. coli GlmU- Complex with UDP-GlcNAc and Acetyl-CoA

Structure of the E. coli bifunctional GlmU acetyltransferase active site with substrates and products.

MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAANELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQL
GTGHAMQQAAPFFADDEDILMLYGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQRLSEVEGVNNRLQLSRLERVYQ
SEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANL
AAACTIGPFARLRPGAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANKFKTIIGDD
VFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWRRPVKKK

Tris, NaCl, DTT, Azide, MgCl2, acetyl-CoA, Glc-1-PO4, UDP-GlcNAc, ammonium sulfate, CoCl2, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K

The biological assembly is trimeric. One trimer is assembled by a crystallographic threefold operation on subunit A. A second trimer is assembled by a crystallographic threefold operation on subunit B. The crystallographic threefold rotation is at (x,y) = (1/3,2/3).