The crystal structure of the molybdenum storage protein from Azotobacter vinelandii loaded with polyoxotungstates (WSto)

Towards Biological Supramolecular Chemistry: A Variety of Pocket-Templated, Individual Metal Oxide Cluster Nucleations in the Cavity of a Mo/W-Storage Protein.

Molybdenum storage protein subunit alpha

Mo storage protein alpha subunit, MoSto subunit alpha

Mo storage protein subunit beta, MoSto subunit beta

(MSE)TDTTNSIKHVISPLARQTLQDRDLTRPVAGKRPIRLLPWLQVVKIGGRV(MSE)DRGADAILPLVEELRKLLPEH
RLLILTGAGVRARHVFSVGLDLGLPVGSLAPLAASEAGQNGHILAA(MSE)LASEGVSYVEHPTVADQLAIHLSATRAVV
GSAFPPYHHHEFPGSRIPPHRADTGAFLLADAFGAAGLTIVENVDGIYTADPNGPDRGQARFLPETSATDLAKSEGPLPV
DRALLDV(MSE)ATARHIERVQVVNGLVPGRLTAALRGEHVGTLIRTGVRPA

MTDTTNSIKHVISPLARQTLQDRDLTRPVAGKRPIRLLPWLQVVKIGGRVMDRGADAILPLVEELRKLLPEHRLLILTGA
GVRARHVFSVGLDLGLPVGSLAPLAASEAGQNGHILAAMLASEGVSYVEHPTVADQLAIHLSATRAVVGSAFPPYHHHEF
PGSRIPPHRADTGAFLLADAFGAAGLTIVENVDGIYTADPNGPDRGQARFLPETSATDLAKSEGPLPVDRALLDVMATAR
HIERVQVVNGLVPGRLTAALRGEHVGTLIRTGVRPA

(MSE)ANSTAELEELL(MSE)QRSLTDPQLQAAAAAAADFRILPDATVIKIGGQSVIDRGRAAVYPLVDEIVAARKNHKL
LIGTGAGTRARHLYSIAAGLGLPAGVLAQLGSSVADQNAA(MSE)LGQLLAKHGIPVVGGAGLSAVPLSLAEVNAVVFSG
(MSE)PPYKLW(MSE)RPAAEGVIPPYRTDAGCFLLAEQFGCKQ(MSE)IFVKDEDGLYTANPKTSKDATFIPRISVDE
(MSE)KAKGLHDSILEFPVLDLLQSAQHVREVQVVNGLVPGNLTRALAGEHVGTIITAS

MANSTAELEELLMQRSLTDPQLQAAAAAAADFRILPDATVIKIGGQSVIDRGRAAVYPLVDEIVAARKNHKLLIGTGAGT
RARHLYSIAAGLGLPAGVLAQLGSSVADQNAAMLGQLLAKHGIPVVGGAGLSAVPLSLAEVNAVVFSGMPPYKLWMRPAA
EGVIPPYRTDAGCFLLAEQFGCKQMIFVKDEDGLYTANPKTSKDATFIPRISVDEMKAKGLHDSILEFPVLDLLQSAQHV
REVQVVNGLVPGNLTRALAGEHVGTIITAS

0.05M MOPS, 0.05M sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The biological assembly is a heterohexamer, (AB)3, generated from the heterodimer AB by the three fold axis: -y+1, x-y, z, and y-x+1, -x+1, z, respectively.

open alpha/beta structure, METAL BINDING PROTEIN

TDTTNSIKHVISPLARQTLQDRDLTRPVAGKRPIRLLPWLQVVKIGGRVMDRGADAILPLVEELRKLLPEHRLLILTGAG
VRARHVFSVGLDLGLPVGSLAPLAASEAGQNGHILAAMLASEGVSYVEHPTVADQLAIHLSATRAVVGSAFPPYHHHEFP
GSRIPPHRADTGAFLLADAFGAAGLTIVENVDGIYTADPNGPDRGQARFLPETSATDLAKSEGPLPVDRALLDVMATARH
IERVQVVNGLVPGRLTAALRGEHVGTLIRTGVRPA

ANSTAELEELLMQRSLTDPQLQAAAAAAADFRILPDATVIKIGGQSVIDRGRAAVYPLVDEIVAARKNHKLLIGTGAGTR
ARHLYSIAAGLGLPAGVLAQLGSSVADQNAAMLGQLLAKHGIPVVGGAGLSAVPLSLAEVNAVVFSGMPPYKLWMRPAAE
GVIPPYRTDAGCFLLAEQFGCKQMIFVKDEDGLYTANPKTSKDATFIPRISVDEMKAKGLHDSILEFPVLDLLQSAQHVR
EVQVVNGLVPGNLTRALAGEHVGTIITAS