Mechanosensitive Channel of Small Conductance (MscS)

Structures of the Prokaryotic Mechanosensitive Channels MscL and MscS

The crystal structure of E. coli MscS, a voltage-modulated and mechanosensitive channel

Small-conductance mechanosensitive channel

MGSSHHHHHHSSGLVPRGSHMEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK
IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGG
VAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDRE
MTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA

PEG 3350, AMMONIUM CHLORIDE, CITRATE, FOSHOLINE-14, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

SEQUENCE
ALL THE NON-HISTIDINE RESIDUES IN THE N-TERMINAL 20
RESIDUES ARE PART OF A CLEAVABLE HIS-TAG CONSTRUCT THAT
WAS ADDED TO THE MSCS SEQUENCE. THE PROTEIN SEQUENCE IS
NOT CLEAVED

The asymmetric unit contains one heptamer which is the biological unit

stretch activated, mechanosensitive ion channel, membrane protein

MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAF
TLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW
SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA