Solution structure of the J domain of EMCV IRES
An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation.
0.5 mM RNA (39-MER), 10 mM potassium phosphate, 10 mM sodium chloride, 100% D2O
0.5 mM RNA (39-MER), 10 mM potassium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O
0.5 mM [U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt RNA (39-MER), 10 mM potassium phosphate, 10 mM sodium chloride, 100% D2O
0.5 mM [U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura RNA (39-MER), 10 mM potassium phosphate, 10 mM sodium chloride, 100% D2O
0.5 mM [U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt RNA (39-MER), 10 mM potassium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O
0.5 mM [U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura RNA (39-MER), 10 mM potassium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore
IRES, translation initiation, Viral RNA, RNA