Solution structure of peptidyl-tRNA hydrolase from Mycobacterium smegmatis
Structural characterization of peptidyl-tRNA hydrolase from Mycobacterium smegmatis by NMR spectroscopy.
MAEPLLVVGLGNPGPTYAKTRHNLGFMVADVLAGRIGSAFKVHKKSGAEVVTGRLAGTSVVLAKPRCYMNESGRQVGPLA KFYSVPPQQIVVIHDELDIDFGRIRLKLGGGEGGHNGLRSVASALGTKNFHRVRIGVGRPPGRKDPAAFVLENFTAAERA EVPTIVEQAADATELLIAQGLEPAQNTVHAW
1 mM [U-100% 15N] Protein, 20 mM sodium phosphate, 50 mM sodium chloride, 0.1 % sodium azide, 1 mM AEBSF protease inhibitor, 1 mM DTT, 95% H2O/5% D2O
1 mM [U-100% 13C; U-100% 15N] Protein, 20 mM sodium phosphate, 50 mM sodium chloride, 0.1 % sodium azide, 1 mM AEBSF protease inhibitor, 1 mM DTT, 95% H2O/5% D2O
1 mM [U-100% 13C; U-100% 15N] Protein, 20 mM sodium phosphate, 50 mM sodium chloride, 0.1 % sodium azide, 1 mM AEBSF protease inhibitor, 1 mM DTT, 100% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Brunger, Adams, Clore, Gros, Nilges and Read