Solution structure of the salicylate-loaded ArCP from yersiniabactin synthetase
Solution Structure of a Nonribosomal Peptide Synthetase Carrier Protein Loaded with Its Substrate Reveals Transient, Well-Defined Contacts.
CHIRALITY ERRORS AT CA ATOM OF RESIDUE 4HJ IN MODELS 12, 15, AND 18.
GTDNRHAADYQQLRERLIQELNLTPQQLHEESNLIQAGLD(4HJ)IRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLM LSRSPE
GTDNRHAADYQQLRERLIQELNLTPQQLHEESNLIQAGLDXIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRS PE
0.3-0.4 mM [U-99% 15N] Loaded-ArCP, 50 mM ACES, 0.5 mM TCEP, 150 mM sodium chloride, 1 mM magnesium chloride, 0.1 mM DSS, 90% H2O/10% D2O
0.3-0.4 mM [U-99% 13C; U-99% 15N] Loaded-ArCP, 50 mM ACES, 0.5 mM TCEP, 150 mM sodium chloride, 1 mM magnesium chloride, 0.1 mM DSS, 2 mM ATP, 2 mM sodium salicylate, 0.02-0.10 uM Adenylation domain YbtE, 90% H2O/10% D2O
0.3-0.4 mM [U-99% 13C; U-99% 15N] Loaded-ArCP, 50 mM ACES, 0.5 mM TCEP, 150 mM sodium chloride, 1 mM magnesium chloride, 0.1 mM DSS, 2 mM ATP, 0.5 mM [U-100% 13C] sodium salicylate, 0.02-0.10 uM Adenylation domain YbtE, 100% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
Carrier Protein, Substrate-Loaded, Nonribosomal Peptide Synthetase, Yersiniabactin, Ligase