Solution structure of holo ArCP from yersiniabactin synthetase
Solution Structure of a Nonribosomal Peptide Synthetase Carrier Protein Loaded with Its Substrate Reveals Transient, Well-Defined Contacts.
GTDNRHAADYQQLRERLIQELNLTPQQLHEESNLIQAGLD(4HH)IRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLM LSRSPE
GTDNRHAADYQQLRERLIQELNLTPQQLHEESNLIQAGLDXIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRS PE
0.3-0.5 mM [U-99% 15N] Holo-ArCP, 50 mM ACES, 0.5 mM TCEP, 150 mM sodium chloride, 1 mM magnesium chloride, 0.1 mM DSS, 90% H2O/10% D2O
0.3-0.5 mM [U-99% 13C; U-99% 15N] Holo-ArCP, 50 mM ACES, 0.5 mM TCEP, 150 mM sodium chloride, 1 mM magnesium chloride, 0.1 mM DSS, 100% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
Carrier Protein, Holo, Nonribosomal Peptide Synthetase, Yersiniabactin, LIGASE