Snu17p-Bud13p structure intermediate during RES complex assembly
Structures of intermediates during RES complex assembly.
core Snu17p, cSnu17p (UNP residues 25-138)
core Bud13p, cBud13p (UNP residues 215-255)
Increased sodium tolerance protein 3, U2 snRNP protein SNU17
Bud site selection protein 13, Complexed with CEF1 protein 26, Synthetic lethal with CLF1 protein 7
GAMGNEYKDNAYIYIGNLNRELTEGDILTVFSEYGVPVDVILSRDENTGESQGFAYLKYEDQRSTILAVDNLNGFKIGGR ALKIDHTFYRPKRSLQKYYEAVKEELDRDIVSKNNAEK
25 mM sodium phosphate, 250 mM sodium chloride, 1 mM sodium azide, 0.8-1 mM [U-13C; U-15N] Snu17p, 1.0-1.2 mM Bud13p, 90% H2O/10% D2O
25 mM sodium phosphate, 250 mM sodium chloride, 1 mM sodium azide, 1-1.2 mM Snu17p, 0.8-1 mM [U-13C; U-15N] Bud13p, 90% H2O/10% D2O
25 mM sodium phosphate, 250 mM sodium chloride, 1 mM sodium azide, 1 mM [U-13C; U-15N] Snu17p, 1 mM [U-13C; U-15N] Bud13p, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
spliceosome, Snu17p, Ist3p, Pml1p, heterodimer, cooperativity, RES, splicing, RRM
NEYKDNAYIYIGNLNRELTEGDILTVFSEYGVPVDVILSRDENTGESQGFAYLKYEDQRSTILAVDNLNGFKIGGRALKI DHTFYRPKRSLQKYYEAVKEELDRDIVSKNNAEK