Solution structure of 53BP1 tandem Tudor domains in complex with a p53K370me2 peptide
Structural Plasticity of Methyllysine Recognition by the Tandem Tudor Domain of 53BP1.
DNA-binding repression region residues 363-377
p53K370Me2, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
GHMNSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSEDEYFSAGVVKG HRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQYGLG
25 mM sodium phosphate, 1.5 mM sodium azide, 2.0 mM [U-100% 13C; U-100% 15N] 53BP1-Tudor, 6.0 mM p53K370me2, 90% H2O/10% D2O
25 mM sodium phosphate, 1.5 mM sodium azide, 2.0 mM [U-100% 13C; U-100% 15N] 53BP1-Tudor, 6.0 mM p53K370me2, 100% D2O
25 mM sodium phosphate, 1.5 mM sodium azide, 5.0 mM 53BP1-Tudor, 2.0 mM [U-100% 13C; U-100% 15N] p53Kc370me2, 90% H2O/10% D2O
25 mM sodium phosphate, 1.5 mM sodium azide, 5.0 mM 53BP1-Tudor, 2.0 mM [U-100% 13C; U-100% 15N] p53Kc370me2, 100% D2O
25 mM sodium phosphate, 1.5 mM sodium azide, 2.0 mM [U-100% 15N] 53BP1-Tudor, 6.0 mM p53K370me2, 90% H2O/10% D2O
35 mM sodium phosphate, 1.5 mM sodium azide, 2.0 mM p53K370me2, 100% D2O
25 mM sodium phosphate, 1.5 mM sodium azide, 2.0 mM [U-100% 13C; U-100% 15N] p53Kc370me2, 100% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, and Kollman
Tumor suppressor p53-binding protein 1, Cellular tumor antigen p53
Cell cycle, DNA damage response, TRANSCRIPTION-ANTITUMOR PROTEIN complex
NSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLGKDILLCDPIPLDTEVTALSEDEYFSAGVVKGHRK ESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQYGLG