Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.
three different labeled samples Ubiquitin-unif [13C-15N] uniformly labeled Ubiquitin-1glc [13C-15N]-1glucose labeled Ubiquitin-2glu [13C-15N]-2glucose labeled
20 mg [U-100% 13C; U-100% 15N] Ubiquitin, 30 mg [1-glucose 13C,U-100% 15N] Ubiquitin, 40 mg [2-glucose 13C,U-100% 15N] Ubiquitin, 40 % v/v MPD, 0.2 M CdCl2, 1 mg DSS, 100% H20
Schwieters, Kuszewski, Tjandra and Clore