Solution structure of the LysM region of the E. coli Intimin periplasmic domain
The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan.
Attaching and effacing protein, Eae protein
MNGENYFKLGSDSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKAAPGQQIILPLKKLP FEYSALPLLGSAPLVAAGGVAGHTNGSGSENLYFQ
simulated annealing, NOESY back-calculation
0.5 mM [U-100% 15N] LysM, 20 mM sodium phosphate, 150 mM sodium chloride, 90% H2O/10% D2O
0.5 mM [U-100% 13C; U-100% 15N] LysM, 20 mM sodium phosphate, 150 mM sodium chloride, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore
ANGENYFKLGSDSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKAAPGQQIILPLKKLP FEYSALPLLGSAPLVAAGGVAGHTN